ArticleProceedings of the National Academy of Sciences of the United States of America2012
Induction of lignocellulose-degrading enzymes in Neurospora crassa by cellodextrins.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 114 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
114 citing papers in PubMed, 234 citations in OpenAlex.
- G-protein signaling components differentially regulate the activity of multiple cellulase enzyme classes in Neurospora crassa.Biotechnology for biofuels and bioproducts · 2026Article
- Intracellular β-glucosidase regulates cellulase expression and development in Aspergillus nidulans.Applied microbiology and biotechnology · 2026Article
- The Zn(II)2Cys6 transcription factorApplied and environmental microbiology · 2026Article
- Heterotrimeric G-proteins and cAMP regulate gene expression during growth on cellulose inmBio · 2026Article
- Xy-31 and Lat-1 regulate the induction of polysaccharide disintegrating genes by arabinose inFrontiers in microbiology · 2026Article
- Selection of reference genes in Thermothelomyces fergusii: insights from genomic and expression analysis in response to different carbon sources.BMC genomics · 2025Article
- Involvement of the intracellular β-glucosidase BGL1B from Aspergillus niger in the regulation of lignocellulose-degrading enzymes' synthesis.Biotechnology for biofuels and bioproducts · 2025Article
- Origins of lineage-specific elements via gene duplication, relocation, and regional rearrangement in Neurospora crassa.Molecular ecology · 2024Article
- Article
- Small GTPase Rab7 is involved in stress adaptation to carbon starvation to ensure the induced cellulase biosynthesis in Trichoderma reesei.Biotechnology for biofuels and bioproducts · 2024Article
- Independent metabolism of oligosaccharides is the keystone of synchronous utilization of cellulose and hemicellulose inPNAS nexus · 2024Article
- Engineering of Ogataea polymorpha strains with ability for high-temperature alcoholic fermentation of cellobiose.FEMS yeast research · 2024Article
- Unveiling a classical mutant in the context of the GH3 β-glucosidase family in Neurospora crassa.AMB Express · 2024Article
- Functional Study of cAMP-Dependent Protein Kinase A inJournal of fungi (Basel, Switzerland) · 2023Article
- Overexpression of the Transcription Factor Azf1 Reveals Novel Regulatory Functions and Impacts β-Glucosidase Production inJournal of fungi (Basel, Switzerland) · 2023Article
- Lineage-specific genes are clustered with HET-domain genes and respond to environmental and genetic manipulations regulating reproduction in Neurospora.PLoS genetics · 2023Article
- Regulation of nutrient utilization in filamentous fungi.Applied microbiology and biotechnology · 2023Review
- Bioproduction and applications of aldobionic acids with a focus on maltobionic and cellobionic acid.Bioprocess and biosystems engineering · 2023Review
- Intracellular Nitric Oxide and cAMP Are Involved in Cellulolytic Enzyme Production inInternational journal of molecular sciences · 2023Article
- Effect of itaconic acid production on Neurospora crassa in consolidated bioprocessing of cellulose.Microbial cell factories · 2023Article
54 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
Abstract
Neurospora crassa colonizes burnt grasslands in the wild and metabolizes both cellulose and hemicellulose from plant cell walls. When switched from a favored carbon source such as sucrose to cellulose, N. crassa dramatically upregulates expression and secretion of a wide variety of genes encoding lignocellulolytic enzymes. However, the means by which N. crassa and other filamentous fungi sense the presence of cellulose in the environment remains unclear. Here, we show that an N. crassa mutant carrying deletions of two genes encoding extracellular β-glucosidase enzymes and one intracellular β-glucosidase lacks β-glucosidase activity, but efficiently induces cellulase gene expression in the presence of cellobiose, cellotriose, or cellotetraose as a sole carbon source. These data indicate that cellobiose, or a modified version of cellobiose, functions as an inducer of lignocellulolytic gene expression in N. crassa. Furthermore, the inclusion of a deletion of the catabolite repressor gene, cre-1, in the triple β-glucosidase mutant resulted in a strain that produces higher concentrations of secreted active cellulases on cellobiose. Thus, the ability to induce cellulase gene expression using a common and soluble carbon source simplifies enzyme production and characterization, which could be applied to other cellulolytic filamentous fungi.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.