ArticleMolecular & cellular proteomics : MCP2012
A highly efficient multifunctional tandem affinity purification approach applicable to diverse organisms.
Article in Molecular & cellular proteomics : MCP, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Isolation, characterization, and mode of action of a class III bacteriocin produced by Lactobacillus helveticus 34.9.World journal of microbiology & biotechnology · 2022Article
- Kinase domain autophosphorylation rewires the activity and substrate specificity of CK1 enzymes.Molecular cell · 2022Article
- Phosphorylation and Pin1 binding to the LIC1 subunit selectively regulate mitotic dynein functions.The Journal of cell biology · 2021Article
- The exocyst complex and Rab5 are required for abscission by localizing ESCRT III subunits to the cytokinetic bridge.Journal of cell science · 2019Article
- The chaperone ERp29 is required for tunneling nanotube formation by stabilizing MSec.The Journal of biological chemistry · 2019Article
- Angiomotins stimulate LATS kinase autophosphorylation and act as scaffolds that promote Hippo signaling.The Journal of biological chemistry · 2018Article
- GSPlant physiology · 2018Article
- An SMC-like protein binds and regulates Caenorhabditis elegans condensins.PLoS genetics · 2017Article
- The Light Intermediate Chain 2 Subpopulation of Dynein Regulates Mitotic Spindle Orientation.Scientific reports · 2016Article
- Intermolecular disulfide bond in the dimerization of S-periaxin mediated by Cys88 and Cys139.Acta biochimica et biophysica Sinica · 2016Article
- An Inducible Retroviral Expression System for Tandem Affinity Purification Mass-Spectrometry-Based Proteomics Identifies Mixed Lineage Kinase Domain-like Protein (MLKL) as an Heat Shock Protein 90 (HSP90) Client.Molecular & cellular proteomics : MCP · 2016Article
- Dynein Light Intermediate Chain 2 Facilitates the Metaphase to Anaphase Transition by Inactivating the Spindle Assembly Checkpoint.PloS one · 2016Article
- Proximity biotinylation and affinity purification are complementary approaches for the interactome mapping of chromatin-associated protein complexes.Journal of proteomics · 2015Article
- An improved toolbox to unravel the plant cellular machinery by tandem affinity purification of Arabidopsis protein complexes.Nature protocols · 2015Article
- Visualization of repetitive DNA sequences in human chromosomes with transcription activator-like effectors.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Mapping and analysis of phosphorylation sites: a quick guide for cell biologists.Molecular biology of the cell · 2013Review
- Challenges and opportunities in the purification of recombinant tagged proteins.Biotechnology advancesReview
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Determining the localization, binding partners, and secondary modifications of individual proteins is crucial for understanding protein function. Several tags have been constructed for protein localization or purification under either native or denaturing conditions, but few tags permit all three simultaneously. Here, we describe a multifunctional tandem affinity purification (MAP) method that is both highly efficient and enables protein visualization. The MAP tag utilizes affinity tags inserted into an exposed surface loop of mVenus offering two advantages: (1) mVenus fluorescence can be used for protein localization or FACS-based selection of cell lines; and (2) spatial separation of the affinity tags from the protein results in high recovery and reduced variability between proteins. MAP purification was highly efficient in multiple organisms for all proteins tested. As a test case, MAP combined with liquid chromatography-tandem MS identified known and new candidate binding partners and modifications of the kinase Plk1. Thus the MAP tag is a new powerful tool for determining protein modification, localization, and interactions.
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Registered trials
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