ArticleThe Journal of physiology2012
Human spermatozoa possess a calcium-dependent chloride channel that may participate in the acrosomal reaction.
Article in The Journal of physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 1 of them a synthesis that pooled it.
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Who cites it
40 citing papers in PubMed, 1 synthesis or guideline pooled it, 66 citations in OpenAlex.
- Sperm Ion Transporters and Channels in Human Asthenozoospermia: Genetic Etiology, Lessons from Animal Models, and Clinical Perspectives.International journal of molecular sciences · 2022Pooled it
- TMEM16A Contributes to Calcium-Activated Chloride Currents and Membrane Potential Changes in the Mouse Oocyte.Journal of cellular physiology · 2026Article
- Chloride Intracellular Channel 4 (CLIC4) controls volume regulation in sperm development via Protein Kinase C (PKC).BMC biology · 2026Article
- Seminal fluid extracellular vesicles restore human sperm osmoregulation after cystic fibrosis transmembrane conductance regulator inhibition.Scientific reports · 2025Article
- Pleiotropic Effects of the NSAID Fenamates on Chloride Channels: Opportunity for Ion Channelopathies?Pharmacology research & perspectives · 2025Review
- TMEM16A Maintains Acrosomal Integrity Through ERK1/2, RhoA, and Actin Cytoskeleton During Capacitation.International journal of molecular sciences · 2025Article
- Evaluating the Protective Effects of MitoQ and Antifreeze Protein III on Cryopreserved Canine Sperm.Animals : an open access journal from MDPI · 2025Article
- Review
- The role of anoctamin 1 in liver disease.Journal of cellular and molecular medicine · 2024Review
- Function and Regulation of the Calcium-Activated Chloride Channel Anoctamin 1 (TMEM16A).Handbook of experimental pharmacology · 2024Article
- Article
- Signaling Roleplay between Ion Channels during Mammalian Sperm Capacitation.Biomedicines · 2023Review
- Electrophysiology of Human Gametes: A Systematic Review.The world journal of men's health · 2022Article
- Perspectives on Potential Fatty Acid Modulations of Motility Associated Human Sperm Ion Channels.International journal of molecular sciences · 2022Review
- The cryopreservation process induces alterations in proteins associated with bull sperm quality: The equilibration process could be a probable critical control point.Frontiers in endocrinology · 2022Article
- Structure and Function of Ion Channels Regulating Sperm Motility-An Overview.International journal of molecular sciences · 2021Review
- Recording Electrical Currents across the Plasma Membrane of Mammalian Sperm Cells.Journal of visualized experiments : JoVE · 2021Article
- Mathematical model reveals that heterogeneity in the number of ion transporters regulates the fraction of mouse sperm capacitation.PloS one · 2021Article
- Human sperm ion channel (dys)function: implications for fertilization.Human reproduction update · 2019Article
- Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Motility, maturation and the acrosome reaction (AR) are fundamental functions of mammalian spermatozoa. While travelling through the female reproductive tract, spermatozoa must mature through a process named capacitation, so that they can reach the egg and undergo the AR, an exocytotic event necessary to fertilize the egg. Though Cl⁻ is important for sperm capacitation and for the AR, not much is known about the molecular identity of the Cl⁻ transporters involved in these processes.We implemented a modified perforated patch-clamp strategy to obtain whole cell recordings sealing on the head of mature human spermatozoa.Our whole cell recordings revealed the presence of a Ca²⁺-dependent Cl⁻ current. The biophysical characteristics of this current and its sensitivity to niflumic acid (NFA) and 4,4-diisothiocyano-2,2-stilbene disulphonic acid (DIDIS) are consistent with those displayed by the Ca²⁺-dependent Cl⁻ channel from the anoctamin family (TMEM16). Whole cell patch clamp recordings in the cytoplasmic droplet of human spermatozoa corroborated the presence of these currents, which were sensitive to NFA and to a small molecule TMEM16A inhibitor (TMEM16Ainh, an aminophenylthiazole). Importantly, the human sperm AR induced by a recombinant human glycoprotein from the zona pellucida, rhZP3, displayed a similar sensitivity to NFA, DIDS and TMEM16Ainh as the sperm Ca²⁺-dependent Cl⁻ currents. Our findings indicate the presence of Ca²⁺-dependent Cl⁻ currents in human spermatozoa, that TMEM16A may contribute to these currents and also that sperm Ca²⁺-dependent Cl⁻ currents may participate in the rhZP3-induced AR.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.