ArticleCell transplantation2012
Isolation of myogenic stem cells from cultures of cryopreserved human skeletal muscle.
Article in Cell transplantation, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 25 citations in OpenAlex.
- Myovascular Niche: The Role of Endothelial Cells in Skeletal Muscle Health and Disease.Circulation research · 2026Review
- Differentiation Capacity of Porcine Skeletal Muscle-Derived Stem Cells as Intermediate Species between Mice and Humans.International journal of molecular sciences · 2023Article
- Tendon stem progenitor cells: Understanding the biology to inform therapeutic strategies for tendon repair.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2019Review
- Coding Cell Identity of Human Skeletal Muscle Progenitor Cells Using Cell Surface Markers: Current Status and Remaining Challenges for Characterization and Isolation.Frontiers in cell and developmental biology · 2019Review
- Article
- Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement.Journal of visualized experiments : JoVE · 2017Article
- Human myocardial pericytes: multipotent mesodermal precursors exhibiting cardiac specificity.Stem cells (Dayton, Ohio) · 2015Article
- Regenerative Translation of Human Blood-Vessel-Derived MSC Precursors.Stem cells international · 2015Review
- Therapeutic isolation and expansion of human skeletal muscle-derived stem cells for the use of muscle-nerve-blood vessel reconstitution.Frontiers in physiology · 2015Article
- Proliferation and differentiation of human adipose-derived mesenchymal stem cells (ASCs) into osteoblastic lineage are passage dependent.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2014Article
- Combined biophysical and soluble factor modulation induces cardiomyocyte differentiation from human muscle derived stem cells.Scientific reports · 2014Article
- Isolation of blood-vessel-derived multipotent precursors from human skeletal muscle.Journal of visualized experiments : JoVE · 2014Article
- Engineered Human Muscle Tissue from Skeletal Muscle Derived Stem Cells and Induced Pluripotent Stem Cell Derived Cardiac Cells.International journal of tissue engineering · 2013Article
- The role of antioxidation and immunomodulation in postnatal multipotent stem cell-mediated cardiac repair.International journal of molecular sciences · 2013Review
- Human myogenic endothelial cells exhibit chondrogenic and osteogenic potentials at the clonal level.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2013Article
- The use of blood vessel-derived stem cells for meniscal regeneration and repair.Medicine and science in sports and exercise · 2013Article
- Cellular kinetics of perivascular MSC precursors.Stem cells international · 2013Review
- Human blood-vessel-derived stem cells for tissue repair and regeneration.Journal of biomedicine & biotechnology · 2012Review
- Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
We demonstrate that subpopulations of adult human skeletal muscle-derived stem cells, myogenic endothelial cells (MECs), and perivascular stem cells (PSCs) can be simultaneously purified by fluorescence-activated cell sorting (FACS) from cryopreserved human primary skeletal muscle cell cultures (cryo-hPSMCs). For FACS isolation, we utilized a combination of cell lineage markers: the myogenic cell marker CD56, the endothelial cell marker UEA-1 receptor (UEA-1R), and the perivascular cell marker CD146. MECs expressing all three cell lineage markers (CD56(+)UEA-1R(+)CD146(+)/CD45(-)) and PSCs expressing only CD146 (CD146(+)/CD45(-)CD56(-)UEA-1R(-)) were isolated by FACS. To evaluate their myogenic capacities, the sorted cells, with and without expansion in culture, were transplanted into the cardiotoxin-injured skeletal muscles of immunodeficient mice. The purified MECs exhibited the highest regenerative capacity in the injured mouse muscles among all cell fractions tested, while PSCs remained superior to myoblasts and the unpurified primary skeletal muscle cells. Our findings show that both MECs and PSCs retain their high myogenic potentials after in vitro expansion, cryopreservation, and FACS sorting. The current study demonstrates that myogenic stem cells are prospectively isolatable from long-term cryopreserved primary skeletal muscle cell cultures. We emphasize the potential application of this new approach to extract therapeutic stem cells from human muscle cells cryogenically banked for clinical purposes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.