ArticleThe Journal of cell biology2012
Migrating fibroblasts reorient directionality by a metastable, PI3K-dependent mechanism.
Article in The Journal of cell biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
68 citing papers in PubMed, 110 citations in OpenAlex.
- Optogenetic control of PLC-γ1 activity directs cell motility.The Journal of cell biology · 2026Article
- The Acidic Exosomal miR-1246/WASF3 Axis Regulates Hepatic Stellate Cell Activation and Stiff ECM Remodeling to Promote Pancreatic Ductal Adenocarcinoma Liver Metastasis.International journal of biological sciences · 2026Article
- Directed migration of cancer cells: a systems biophysics perspective.Frontiers in cell and developmental biology · 2026Review
- Optogenetic control of PLC-γ1 activity polarizes cell motility.bioRxiv : the preprint server for biology · 2025Article
- FAK Differentially Mechanoregulates Cell Migration during Wound Closure.ACS biomaterials science & engineering · 2025Article
- FAK Differentially Mechanoregulates Cell Migration During Wound Closure.bioRxiv : the preprint server for biology · 2025Article
- Spatiotemporal analysis of F-actin polymerization with micropillar arrays reveals synchronization between adhesion sites.Molecular biology of the cell · 2024Article
- Single-cell transcriptomics reveal distinctive patterns of fibroblast activation in heart failure with preserved ejection fraction.Basic research in cardiology · 2024Article
- Proteolysis-free amoeboid migration of melanoma cells through crowded environments via bleb-driven worrying.Developmental cell · 2024Article
- Quantifying collective motion patterns in mesenchymal cell populations using topological data analysis and agent-based modeling.Mathematical biosciences · 2024Article
- Non-catalytic role of phosphoinositide 3-kinase in mesenchymal cell migration through non-canonical induction of p85β/AP2-mediated endocytosis.Nature communications · 2024Article
- Particle-based simulations reveal two positive feedback loops allow relocation and stabilization of the polarity site during yeast mating.PLoS computational biology · 2023Article
- Crawling, waving, inch worming, dilating, and pivoting mechanics of migrating cells: Lessons from Ken Jacobson.Biophysical journal · 2023Review
- Non-asymptotic transients away from steady states determine cellular responsiveness to dynamic spatial-temporal signals.PLoS computational biology · 2023Article
- Random walk and cell morphology dynamics inFrontiers in cell and developmental biology · 2023Article
- Unified multiscale theory of cellular mechanical adaptations to substrate stiffness.Biophysical journal · 2022Article
- Understanding the Role of Fibroblasts following a 3D Tumoroid Implantation for Breast Tumor Formation.Bioengineering (Basel, Switzerland) · 2021Review
- The principles of directed cell migration.Nature reviews. Molecular cell biology · 2021Review
- Microfluidic devices fitted with "flowver" paper pumps generate steady, tunable gradients for extended observation of chemotactic cell migration.Biomicrofluidics · 2021Article
- A novel stochastic simulation approach enables exploration of mechanisms for regulating polarity site movement.PLoS computational biology · 2021Article
8 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Mesenchymal cell migration as exhibited by fibroblasts is distinct from amoeboid cell migration and is characterized by dynamic competition among multiple protrusions, which determines directional persistence and responses to spatial cues. Localization of phosphoinositide 3-kinase (PI3K) signaling is thought to play a broadly important role in cell motility, yet the context-dependent functions of this pathway have not been adequately elucidated. By mapping the spatiotemporal dynamics of cell protrusion/retraction and PI3K signaling monitored by total internal reflection fluorescence microscopy, we show that randomly migrating fibroblasts reorient polarity through PI3K-dependent branching and pivoting of protrusions. PI3K inhibition did not affect the initiation of newly branched protrusions, nor did it prevent protrusion induced by photoactivation of Rac. Rather, PI3K signaling increased after, not before, the onset of local protrusion and was required for the lateral spreading and stabilization of nascent branches. During chemotaxis, the branch experiencing the higher chemoattractant concentration was favored, and, thus, the cell reoriented so as to align with the external gradient.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.