ArticleJournal of molecular biology2012
An examination of the electrostatic interactions between the N-terminal tail of the Brome Mosaic Virus coat protein and encapsidated RNAs.
Article in Journal of molecular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
43 citing papers in PubMed, 85 citations in OpenAlex.
- Electrostatic Theory of the Acidity of the Solution in the Lumina of Viruses and Virus-Like Particles.The journal of physical chemistry. B · 2023Article
- Single-particle studies of the effects of RNA-protein interactions on the self-assembly of RNA virus particles.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Variability among the Isolates of Broad Bean Mottle Virus and Encapsidation of Host RNAs.Pathogens (Basel, Switzerland) · 2022Article
- Applications of Charge Detection Mass Spectrometry in Molecular Biology and Biotechnology.Chemical reviews · 2022Review
- Packaging contests between viral RNA molecules and kinetic selectivity.PLoS computational biology · 2022Article
- Virus Assembly Pathways: Straying Away but Not Too Far.Small (Weinheim an der Bergstrasse, Germany) · 2020Article
- Theoretical Modeling of Chemical Equilibrium in Weak Polyelectrolyte Layers on Curved Nanosystems.Polymers · 2020Review
- Physical virology: From virus self-assembly to particle mechanics.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2020Review
- Genome organization and interaction with capsid protein in a multipartite RNA virus.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Electrostatics-Driven Inflation of Elastic Icosahedral Shells as a Model for Swelling of Viruses.Biophysical journal · 2018Article
- Systematic analysis of biological roles of charged amino acid residues located throughout the structured inner wall of a virus capsid.Scientific reports · 2018Article
- The different faces of mass action in virus assembly.Journal of biological physics · 2018Article
- Self consistent field theory of virus assembly.Journal of physics. Condensed matter : an Institute of Physics journal · 2018Article
- Intrinsically-disordered N-termini in human parechovirus 1 capsid proteins bind encapsidated RNA.Scientific reports · 2018Article
- Compactness of viral genomes: effect of disperse and localized random mutations.Journal of physics. Condensed matter : an Institute of Physics journal · 2018Article
- The effect of RNA stiffness on the self-assembly of virus particles.Journal of physics. Condensed matter : an Institute of Physics journal · 2018Article
- Varieties of charge distributions in coat proteins of ssRNA+ viruses.Journal of physics. Condensed matter : an Institute of Physics journal · 2018Article
- The intrinsically disordered N-terminal arm of the brome mosaic virus coat protein specifically recognizes the RNA motif that directs the initiation of viral RNA replication.Nucleic acids research · 2018Article
- Length of encapsidated cargo impacts stability and structure of in vitro assembled alphavirus core-like particles.Journal of physics. Condensed matter : an Institute of Physics journal · 2017Article
- The Effect of RNA Secondary Structure on the Self-Assembly of Viral Capsids.Biophysical journal · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
The coat protein of positive-stranded RNA viruses often contains a positively charged tail that extends toward the center of the capsid and interacts with the viral genome. Electrostatic interaction between the tail and the RNA has been postulated as a major force in virus assembly and stabilization. The goal of this work is to examine the correlation between electrostatic interaction and amount of RNA packaged in the tripartite Brome Mosaic Virus (BMV). Nanoindentation experiment using atomic force microscopy showed that the stiffness of BMV virions with different RNAs varied by a range that is 10-fold higher than that would be predicted by electrostatics. BMV mutants with decreased positive charges encapsidated lower amounts of RNA while mutants with increased positive charges packaged additional RNAs up to ∼900 nt. However, the extra RNAs included truncated BMV RNAs, an additional copy of RNA4, potential cellular RNAs, or a combination of the three, indicating that change in the charge of the capsid could result in several different outcomes in RNA encapsidation. In addition, mutant with specific arginines changed to lysines in the capsid also exhibited defects in the specific encapsidation of BMV RNA4. The experimental results indicate that electrostatics is a major component in RNA encapsidation but was unable to account for all of the observed effects on RNA encapsidation. Thermodynamic modeling incorporating the electrostatics was able to predict the approximate length of the RNA to be encapsidated for the majority of mutant virions, but not for a mutant with extreme clustered positive charges. Cryo-electron microscopy of virions that encapsidated an additional copy of RNA4 revealed that, despite the increase in RNA encapsidated, the capsid structure was minimally changed. These results experimentally demonstrated the impact of electrostatics and additional restraints in the encapsidation of BMV RNAs, which could be applicable to other viruses.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.