ArticleJournal of the American Chemical Society2012
Minimalist probes for studying protein dynamics: thioamide quenching of selectively excitable fluorescent amino acids.
Article in Journal of the American Chemical Society, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 82 citations in OpenAlex.
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- Highly Improved Sensitivity of FET Sensors Based on a Pulsed Temperature Profile for Lead Ion Detection.Analytical chemistry · 2026Article
- A Grignard-mediated route to access 4-substituted-3-RSC advances · 2026Article
- Expanding the fluorescence toolkit: molecular design, synthesis and biological application of unnatural amino acids.Chemical science · 2025Review
- Improved Large-Scale Synthesis of Acridonylalanine for Diverse Peptide and Protein Applications.Bioconjugate chemistry · 2024Article
- Critical assessment of selenourea as an efficient small molecule fluorescence quenching probe to monitor protein dynamics.Chemical science · 2023Article
- Continuous Histone Deacylase Activity Assays.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Structural impact of thioamide incorporation into a β-hairpin.RSC chemical biology · 2022Article
- Expansion of the Genetic Code Through the Use of Modified Bacterial Ribosomes.Journal of molecular biology · 2022Review
- Incorporating thioamides into proteins by native chemical ligation.Methods in enzymology · 2021Article
- Facile synthesis of a novel genetically encodable fluorescent α-amino acid emitting greenish blue light.Chemical communications (Cambridge, England) · 2020Article
- Coumarin as a structural component of substrates and probes for serine and cysteine proteases.Biochimica et biophysica acta. Proteins and proteomics · 2020Review
- Fluorescently Labelled ATP Analogues for Direct Monitoring of Ubiquitin Activation.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020Article
- Single-Atom Fluorescence Switch: A General Approach toward Visible-Light-Activated Dyes for Biological Imaging.Journal of the American Chemical Society · 2019Article
- 4-Cyanoindole-2'-deoxyribonucleoside as a Dual Fluorescence and Infrared Probe of DNA Structure and Dynamics.Molecules (Basel, Switzerland) · 2019Article
- Improving target amino acid selectivity in a permissive aminoacyl tRNA synthetase through counter-selection.Organic & biomolecular chemistry · 2017Article
- The effects of thioamide backbone substitution on protein stability: a study in α-helical, β-sheet, and polyproline II helical contexts.Chemical science · 2017Article
- Minimalist IR and fluorescence probes of protein function.Current opinion in chemical biology · 2016Review
- Chemoselective modifications for the traceless ligation of thioamide-containing peptides and proteins.Organic & biomolecular chemistry · 2016Article
- Electronic interactions of i, i + 1 dithioamides: increased fluorescence quenching and evidence for n-to-π* interactions.Chemical communications (Cambridge, England) · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Fluorescent probe pairs that can be selectively excited in the presence of Trp and Tyr are of great utility in studying conformational changes in proteins. However, the size of these probe pairs can restrict their incorporation to small portions of a protein sequence where their effects on secondary and tertiary structure can be tolerated. Our findings show that a thioamide bond-a single atom substitution of the peptide backbone-can quench fluorophores that are red-shifted from intrinsic protein fluorescence, such as acridone. Using steady-state and fluorescence lifetime measurements, we further demonstrate that this quenching occurs through a dynamic electron-transfer mechanism. In a proof-of-principle experiment, we apply this technique to monitor unfolding in a model peptide system, the villin headpiece HP35 fragment. Thioamide analogues of the natural amino acids can be placed in a variety of locations in a protein sequence, allowing one to make a large number of measurements to model protein folding.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.