ArticleMolecular and cellular biology1990
Two adjacent C/EBP-binding sequences that participate in the cell-specific expression of the mouse serum amyloid A3 gene.
Article in Molecular and cellular biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 51 citations in OpenAlex.
- Liver transcriptome profiles associated with strain-specific Ehrlichia chaffeensis-induced hepatitis in SCID mice.Infection and immunity · 2009Article
- Effects of hepatocyte nuclear factor-4alpha on the regulation of the hepatic acute phase response.Journal of molecular biology · 2007Article
- Tissue-specific regulatory elements in mammalian promoters.Molecular systems biology · 2007Article
- Regulation of serum amyloid A protein expression during the acute-phase response.The Biochemical journal · 1998Review
- Stress-induced binding of the transcriptional factor CHOP to a novel DNA control element.Molecular and cellular biology · 1996Article
- Inhibition of cell proliferation by C/EBP alpha occurs in many cell types, does not require the presence of p53 or Rb, and is not affected by large T-antigen.Nucleic acids research · 1995Article
- Multiple initiators and C/EBP binding sites are involved in transcription from the TATA-less rat XDH/XO basal promoter.Nucleic acids research · 1995Article
- Induction of the mouse serum amyloid A3 gene by cytokines requires both C/EBP family proteins and a novel constitutive nuclear factor.Molecular and cellular biology · 1994Article
- Serum amyloid A gene expression under acute-phase conditions involves participation of inducible C/EBP-beta and C/EBP-delta and their activation by phosphorylation.Molecular and cellular biology · 1994Article
- Molecular analysis of the differential hepatic expression of rat kininogen family genes.Molecular and cellular biology · 1993Article
- Differentiated liver cell specificity of the second enhancer of hepatitis B virus.Journal of virology · 1993Article
- Analysis of the promoter element of the serum amyloid A gene and its interaction with constitutive and inducible nuclear factors from rabbit liver.Gene expression · 1993Article
- Cooperative effects of C/EBP-like and NF kappa B-like binding sites on rat serum amyloid A1 gene expression in liver cells.Nucleic acids research · 1992Article
- Novel DNA-binding proteins regulate intestine-specific transcription of the sucrase-isomaltase gene.Molecular and cellular biology · 1992Article
- C/EBP-like proteins binding to the functional box-alpha and box-beta of the second enhancer of hepatitis B virus.Molecular and cellular biology · 1991Article
- Genome organization and expression of the rat ACBP gene family.Molecular and cellular biochemistryArticle
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Serum amyloid A (SAA) is a major acute-phase protein synthesized primarily in the liver. Its expression, very low in normal animals, is increased several hundredfold following acute inflammation. To examine DNA sequences involved in liver-specific expression, 5'-flanking regions of the mouse SAA3 gene were analyzed by transient transfection, band shift, and DNase I protection assays. We found that a 56-bp fragment immediately 5' to the TATA box spanning the region -93 to -38 relative to the transcription start site was sufficient to confer liver cell-specific transcriptional activation onto a heterologous promoter in a dose-dependent and orientation-independent manner. This DNA fragment could form DNA-protein complexes with heat-stable nuclear proteins, and the complexes formed could be specifically competed for by excess oligomers corresponding to the C/EBP- or DBP-binding sites but not by binding sites for three other liver-specific factors, HNF1, HNF3, and HNF4. Footprint analysis using Hep3B nuclear extracts revealed two adjacent footprint regions within this 56-bp fragment, the distal region having at least fivefold-greater affinity than the proximal region. Identical footprint patterns were observed when purified recombinant C/EBP protein was used. These results indicated that binding of C/EBP to this 56-bp fragment plays an important role in vivo in enhancing expression of the mouse SAA3 gene in hepatocytes.
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