ArticleFrontiers in cellular neuroscience2012
Neurons derived from human embryonic stem cells extend long-distance axonal projections through growth along host white matter tracts after intra-cerebral transplantation.
Article in Frontiers in cellular neuroscience, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Stem cell transplantation in traumatic spinal cord injury: a systematic review and meta-analysis of animal studies.PLoS biology · 2013Pooled it
- Age-dependent integration of cortical progenitors transplanted at CSF-neurogenic niche interface.Frontiers in cell and developmental biology · 2025Article
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- Advances in human stem cell therapies: pre-clinical studies and the outlook for central nervous system regeneration.Neural regeneration research · 2021Article
- Activity in grafted human iPS cell-derived cortical neurons integrated in stroke-injured rat brain regulates motor behavior.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Intracerebral Transplants of GMP-Grade Human Umbilical Cord-Derived Mesenchymal Stromal Cells Effectively Treat Subacute-Phase Ischemic Stroke in a Rodent Model.Frontiers in cellular neuroscience · 2020Article
- Harnessing stem cells and biomaterials to promote neural repair.British journal of pharmacology · 2019Review
- Grafted Human iPSC-Derived Neural Progenitor Cells Express Integrins and Extend Long-Distance Axons Within the Developing Corticospinal Tract.Frontiers in cellular neuroscience · 2019Article
- A PITX3-EGFP Reporter Line Reveals Connectivity of Dopamine and Non-dopamine Neuronal Subtypes in Grafts Generated from Human Embryonic Stem Cells.Stem cell reports · 2017Article
- Long-Distance Axonal Growth and Protracted Functional Maturation of Neurons Derived from Human Induced Pluripotent Stem Cells After Intracerebral Transplantation.Stem cells translational medicine · 2017Article
- Anatomically Inspired Three-dimensional Micro-tissue Engineered Neural Networks for Nervous System Reconstruction, Modulation, and Modeling.Journal of visualized experiments : JoVE · 2017Article
- Neuronal replacement therapy: previous achievements and challenges ahead.NPJ Regenerative medicine · 2017Review
- WDR62 Regulates Early Neural and Glial Progenitor Specification of Human Pluripotent Stem Cells.Stem cells international · 2017Article
- Advanced biomaterial strategies to transplant preformed micro-tissue engineered neural networks into the brain.Journal of neural engineering · 2016Article
- Rebuilding Brain Circuitry with Living Micro-Tissue Engineered Neural Networks.Tissue engineering. Part A · 2015Article
- The nuclear localization pattern and interaction partners of GTF2IRD1 demonstrate a role in chromatin regulation.Human genetics · 2015Article
- Functional recovery after acute intravenous administration of human umbilical cord mesenchymal stem cells in rats with cerebral ischemia-reperfusion injury.Intractable & rare diseases research · 2015Article
- Human ESC-derived dopamine neurons show similar preclinical efficacy and potency to fetal neurons when grafted in a rat model of Parkinson's disease.Cell stem cell · 2014Article
- Neonatal immune-tolerance in mice does not prevent xenograft rejection.Experimental neurology · 2014Article
- Transplantation of bone marrow stromal cells enhances infiltration and survival of CNP and Schwann cells to promote axonal sprouting following complete transection of spinal cord in adult rats.American journal of translational research · 2014Article
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8 authors.
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Abstract
Human pluripotent stem cells have the capacity for directed differentiation into a wide variety of neuronal subtypes that may be useful for brain repair. While a substantial body of research has lead to a detailed understanding of the ability of neurons in fetal tissue grafts to structurally and functionally integrate after intra-cerebral transplantation, we are only just beginning to understand the in vivo properties of neurons derived from human pluripotent stem cells. Here we have utilized the human embryonic stem (ES) cell line Envy, which constitutively expresses green fluorescent protein (GFP), in order to study the in vivo properties of neurons derived from human ES cells. Rapid and efficient neural induction, followed by differentiation as neurospheres resulted in a GFP+ neural precursor population with traits of neuroepithelial and dorsal forebrain identity. Ten weeks after transplantation into neonatal rats, GFP+ fiber patterns revealed extensive axonal growth in the host brain, particularly along host white matter tracts, although innervation of adjacent nuclei was limited. The grafts were composed of a mix of neural cell types including differentiated neurons and glia, but also dividing neural progenitors and migrating neuroblasts, indicating an incomplete state of maturation at 10 weeks. This was reflected in patch-clamp recordings showing stereotypical properties appropriate for mature functional neurons, including the ability to generate action potentials, as well profiles consistent for more immature neurons. These findings illustrate the intrinsic capacity for neurons derived from human ES cells to integrate at a structural and functional level following transplantation.
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