ArticleCurrent protocols in neuroscience2012
Culturing embryonic nasal explants for developmental and physiological study.
Article in Current protocols in neuroscience, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Mild Gestational Hypothyroidism in Mice Has Transient Developmental Effects and Long-Term Consequences on Neuroendocrine Systems.Thyroid : official journal of the American Thyroid Association · 2025Article
- Disruption of Intranasal GnRH Neuronal Migration Route into the Brain Induced by Proinflammatory Cytokine IL-6: Ex Vivo and In Vivo Rodent Models.International journal of molecular sciences · 2023Article
- Article
- Nasal Placode Development, GnRH Neuronal Migration and Kallmann Syndrome.Frontiers in cell and developmental biology · 2019Article
- GPR37 Signaling Modulates Migration of Olfactory Ensheathing Cells and Gonadotropin Releasing Hormone Cells in Mice.Frontiers in cellular neuroscience · 2019Article
- Reelin Can Modulate Migration of Olfactory Ensheathing Cells and Gonadotropin Releasing Hormone Neurons via the Canonical Pathway.Frontiers in cellular neuroscience · 2018Article
- Galanin Activates G Protein Gated Inwardly Rectifying Potassium Channels and Suppresses Kisspeptin-10 Activation of GnRH Neurons.Endocrinology · 2016Article
- BPA Directly Decreases GnRH Neuronal Activity via Noncanonical Pathway.Endocrinology · 2016Article
- Chloride Accumulators NKCC1 and AE2 in Mouse GnRH Neurons: Implications for GABAA Mediated Excitation.PloS one · 2015Article
- Metabolic influences on reproduction: adiponectin attenuates GnRH neuronal activity in female mice.Endocrinology · 2014Article
- Capture of microtubule plus-ends at the actin cortex promotes axophilic neuronal migration by enhancing microtubule tension in the leading process.Frontiers in cellular neuroscience · 2014Article
- The indirect role of fibroblast growth factor-8 in defining neurogenic niches of the olfactory/GnRH systems.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Calcium release-dependent actin flow in the leading process mediates axophilic migration.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Embed dynamic content in your poster.Science signaling · 2013Article
- SDF and GABA interact to regulate axophilic migration of GnRH neurons.Journal of cell science · 2012Article
- The Dopamine D4 Receptor Regulates Gonadotropin-Releasing Hormone Neuron Excitability in Male Mice.eNeuroArticle
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Authors and funding
2 authors.
Funding
Abstract
Primary cultures obtained from embryonic nasal placodes can maintain olfactory neurons, olfactory ensheathing cells, and large numbers of gonadotropin releasing hormone-1 (GnRH) neurons. Depending on the age of the starting material, one can examine cell interactions important for placode formation or neuronal migration and axonal outgrowth. When generated at E11.5 in mouse, neuronal migration and axon outgrowth away from the main tissue mass occurs. This area of the explant, the periphery, is only a few cells thick. This characteristic offers the opportunity to image single cells and axons and allows pharmacological and molecular manipulations as well as physiological recordings to be performed. Here, we describe a system for culturing nasal explants used in our laboratory. This model system provides a method for obtaining physiological cellular responses with post hoc immunohistochemistry, or gene expression studies, on cells arising from the nasal placode.
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