ArticleMolecular microbiology2012
Characterization of the Group A Streptococcus Mga virulence regulator reveals a role for the C-terminal region in oligomerization and transcriptional activation.
Article in Molecular microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 26 citations in OpenAlex.
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- Promoter DNA recognition by theFrontiers in molecular biosciences · 2023Article
- Slipped-strand mispairing within a polycytidine tract in transcriptional regulatorFrontiers in microbiology · 2023Article
- Carbon catabolite repression on the Rgg2/3 quorum sensing system in Streptococcus pyogenes is mediated by PTSMolecular microbiology · 2022Article
- Review
- PRD-Containing Virulence Regulators (PCVRs) in Pathogenic Bacteria.Frontiers in cellular and infection microbiology · 2021Review
- DNA-binding properties of MafR, a global regulator of Enterococcus faecalis.FEBS letters · 2018Article
- Regulons and protein-protein interactions of PRD-containing Bacillus anthracis virulence regulators reveal overlapping but distinct functions.Molecular microbiology · 2018Article
- Glucose Levels Alter the Mga Virulence Regulon in the Group A Streptococcus.Scientific reports · 2018Article
- Multimerization of the Virulence-Enhancing Group A Streptococcus Transcription Factor RivR Is Required for Regulatory Activity.Journal of bacteriology · 2017Article
- Genomic Comparison among Lethal Invasive Strains ofFrontiers in microbiology · 2017Article
- Genomic and transcriptomic insights into the efficient entomopathogenicity of Bacillus thuringiensis.Scientific reports · 2015Article
- Phosphorylation events in the multiple gene regulator of group A Streptococcus significantly influence global gene expression and virulence.Infection and immunity · 2015Article
- Streptococcus pyogenes malate degradation pathway links pH regulation and virulence.Infection and immunity · 2015Article
- Crystal structure of Bacillus anthracis virulence regulator AtxA and effects of phosphorylated histidines on multimerization and activity.Molecular microbiology · 2015Article
- The pneumococcal MgaSpn virulence transcriptional regulator generates multimeric complexes on linear double-stranded DNA.Nucleic acids research · 2013Article
- PTS phosphorylation of Mga modulates regulon expression and virulence in the group A streptococcus.Molecular microbiology · 2013Article
- Nucleotides critical for the interaction of the Streptococcus pyogenes Mga virulence regulator with Mga-regulated promoter sequences.Journal of bacteriology · 2012Article
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6 authors at 1 institution in 1 country.
Funding
Abstract
The Group A Streptococcus (GAS) is a strict human pathogen that causes a broad spectrum of illnesses. One of the key regulators of virulence in GAS is the transcriptional activator Mga, which co-ordinates the early stages of infection. Although the targets of Mga have been well characterized, basic biochemical analyses have been limited due to difficulties in obtaining purified protein. In this study, high-level purification of soluble Mga was achieved, enabling the first detailed characterization of the protein. Fluorescence titrations coupled with filter-binding assays indicate that Mga binds cognate DNA with nanomolar affinity. Gel filtration analyses, analytical ultracentrifugation and co-immunoprecipitation experiments demonstrate that Mga forms oligomers in solution.Moreover, the ability of the protein to oligomerize in solution was found to correlate with transcriptional activation; DNA binding appears to be necessary but insufficient for full activity. Truncation analyses reveal that the uncharacterized C-terminal region of Mga, possessing similarity to phosphotransferase system EIIB proteins, plays a critical role in oligomerization and in vivo activity. Mga from a divergent serotype was found to behave similarly, suggesting that this study describes a general mechanism for Mga regulation of target virulence genes within GAS and provides insight into related regulators in other Gram-positive pathogens.
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