ArticleJournal of cell science2012
PF19 encodes the p60 catalytic subunit of katanin and is required for assembly of the flagellar central apparatus in Chlamydomonas.
Article in Journal of cell science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 45 citations in OpenAlex.
- KATANIN promotes cell elongation and division to generate proper cell numbers in maize organs.Nature communications · 2026Article
- The Small Interactor of PKD2 protein promotes the assembly and ciliary entry of the Chlamydomonas PKD2-mastigoneme complexes.Journal of cell science · 2024Article
- Combinatorial and antagonistic effects of tubulin glutamylation and glycylation on katanin microtubule severing.Developmental cell · 2022Article
- Wdr47, Camsaps, and Katanin cooperate to generate ciliary central microtubules.Nature communications · 2021Article
- Composition and function of the C1b/C1f region in the ciliary central apparatus.Scientific reports · 2021Article
- Central Apparatus, the Molecular Kickstarter of Ciliary and Flagellar Nanomachines.International journal of molecular sciences · 2021Review
- Asymmetries in the cilia ofPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Review
- Review
- Primary cilium loss in mammalian cells occurs predominantly by whole-cilium shedding.PLoS biology · 2019Article
- Proteome of the central apparatus of a ciliary axoneme.The Journal of cell biology · 2019Article
- Article
- Microtubule-severing enzymes: From cellular functions to molecular mechanism.The Journal of cell biology · 2018Review
- Review
- Posttranslational Modifications of Tubulin and Cilia.Cold Spring Harbor perspectives in biology · 2017Review
- Chromosome-level genome assembly and transcriptome of the green algaProceedings of the National Academy of Sciences of the United States of America · 2017Article
- The Central Apparatus of Cilia and Eukaryotic Flagella.Cold Spring Harbor perspectives in biology · 2017Review
- Proteomic Analysis of the Mammalian Katanin Family of Microtubule-severing Enzymes Defines Katanin p80 subunit B-like 1 (KATNBL1) as a Regulator of Mammalian Katanin Microtubule-severing.Molecular & cellular proteomics : MCP · 2016Article
- The basal bodies of Chlamydomonas reinhardtii.Cilia · 2016Review
- A novel family of katanin-like 2 protein isoforms (KATNAL2), interacting with nucleotide-binding proteins Nubp1 and Nubp2, are key regulators of different MT-based processes in mammalian cells.Cellular and molecular life sciences : CMLS · 2016Article
- KIAA0556 is a novel ciliary basal body component mutated in Joubert syndrome.Genome biology · 2015Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
For all eukaryotic cilia the basal bodies provide a template for the assembly of the doublet microtubules, and intraflagellar transport provides a mechanism for transport of axonemal components into the growing cilium. What is not known is how the central pair of microtubules is nucleated or how their associated polypeptides are assembled. Here we report that the Chlamydomonas pf19 mutation results in a single amino acid change within the p60 catalytic subunit of katanin, and that this mutation prevents microtubule severing activity. The pf19 mutant has paralyzed flagella that lack the central apparatus. Using a combination of mutant analysis, RNAi-mediated reduction of protein expression and in vitro assays, we demonstrate that the p60 catalytic subunit of the microtubule severing protein katanin is required for central apparatus assembly in Chlamydomonas. In addition, we show that in Chlamydomonas the microtubule severing activity of p60 katanin is not required for stress-induced deflagellation or cell cycle progression as has been previously reported.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.