ArticleInvestigative ophthalmology & visual science2012
Quantitative assessment of ultrastructure and light scatter in mouse corneal debridement wounds.
Article in Investigative ophthalmology & visual science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 65 citations in OpenAlex.
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- Ultrastructural Aspects of Corneal Functional Recovery in Rats Following Intrastromal Keratocyte Injection.Investigative ophthalmology & visual science · 2025Article
- Impact of keratocyte differentiation on corneal opacity resolution and visual function recovery in male rats.Nature communications · 2024Article
- Animal Models for Limbal Stem Cell Deficiency: A Critical Narrative Literature Review.Ophthalmology and therapy · 2024Review
- Posterior corneoscleral limbus: Architecture, stem cells, and clinical implications.Progress in retinal and eye research · 2023Review
- Assessment of Keratitis Severity Using Quantitative Image Analysis in an In Vivo Murine Model of Staphylococcus aureus Bacterial Keratitis.Translational vision science & technology · 2022Article
- Recent advances in optical coherence tomography for anterior segment imaging in small animals and their clinical implications.The ocular surface · 2022Review
- ECM stiffness modulates the proliferation but not the motility of primary corneal keratocytes in response to PDGF-BB.Experimental eye research · 2022Article
- Regenerative therapy for the Cornea.Progress in retinal and eye research · 2022Review
- Signaling Downstream of Focal Adhesions Regulates Stiffness-Dependent Differences in the TGF-Frontiers in cell and developmental biology · 2022Article
- Spectral Transmission of the Human Corneal Layers.Journal of clinical medicine · 2021Article
- ECM Stiffness Controls the Activation and Contractility of Corneal Keratocytes in Response to TGF-β1.Biophysical journal · 2020Article
- Therapeutic Potential of Extracellular Vesicles for the Treatment of Corneal Injuries and Scars.Translational vision science & technology · 2020Article
- Keratocyte mechanobiology.Experimental eye research · 2020Review
- A Novel CD147 Inhibitor, SP-8356, Attenuates Pathological Fibrosis in Alkali-Burned Rat Cornea.International journal of molecular sciences · 2020Article
- Development of Transparent Acellular Dermal Matrix as Tissue-Engineered Stroma Substitute for Central Lamellar Keratoplasty.Investigative ophthalmology & visual science · 2020Article
- Mesenchymal Stem Cells Reduce Corneal Fibrosis and Inflammation via Extracellular Vesicle-Mediated Delivery of miRNA.Stem cells translational medicine · 2019Article
- Assessment of Corneal Stromal Remodeling and Regeneration after Photorefractive Keratectomy.Scientific reports · 2018Article
- Corneal Epithelial Abrasion with Ocular Burr As a Model for Cornea Wound Healing.Journal of visualized experiments : JoVE · 2018Article
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 2 countries.
Funding
Abstract
purposeThe mouse has become an important wound healing model with which to study corneal fibrosis, a frequent complication of refractive surgery. The aim of the current study was to quantify changes in stromal ultrastructure and light scatter that characterize fibrosis in mouse corneal debridement wounds.
methodsEpithelial debridement wounds, with and without removal of basement membrane, were produced in C57BL/6 mice. Corneal opacity was measured using optical coherence tomography, and collagen diameter and matrix order were quantified by x-ray scattering. Electron microscopy was used to visualize proteoglycans. Quantitative PCR (Q-PCR) measured mRNA transcript levels for several quiescent and fibrotic markers.
resultsEpithelial debridement without basement membrane disruption produced a significant increase in matrix disorder at 8 weeks, but minimal corneal opacity. In contrast, basement membrane penetration led to increases in light scatter, matrix disorder, and collagen diameter, accompanied by the appearance of abnormally large proteoglycans in the subepithelial stroma. This group also demonstrated upregulation of several quiescent and fibrotic markers 2 to 4 weeks after wounding.
conclusionsFibrotic corneal wound healing in mice involves extensive changes to collagen and proteoglycan ultrastructure, consistent with deposition of opaque scar tissue. Epithelial basement membrane penetration is a deciding factor determining the degree of ultrastructural changes and resulting opacity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.