ArticleInvestigative ophthalmology & visual science2012
Analysis of human adenovirus type 19 associated with epidemic keratoconjunctivitis and its reclassification as adenovirus type 64.
Article in Investigative ophthalmology & visual science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers, 2 of them syntheses that pooled it.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
60 citing papers in PubMed, 2 syntheses or guidelines pooled it, 96 citations in OpenAlex.
- High case fatality rate of human adenovirus type 7 infections in children: a meta-analysis and phylogenetic study of HAdV-7 epidemics over the past seven decades.Emerging microbes & infections · 2025Pooled it
- Topical pharmacologic interventions versus placebo for epidemic keratoconjunctivitis.The Cochrane database of systematic reviews · 2022Pooled it
- Molecular and Clinical Characterization of Human Adenovirus E4-Associated Conjunctivitis.American journal of ophthalmology · 2022Trial
- Genotypes and phylogenetic analysis of adenovirus in children with respiratory infection in Buenos Aires, Argentina (2000-2018).PloS one · 2021Trial
- Determinants of Outcomes of Adenoviral Keratoconjunctivitis.Ophthalmology · 2018Trial
- Review
- Evaluation of factors relating to the development of multiple subepithelial corneal infiltrates of adenoviral keratoconjunctivitis using categorical data analysis program.Japanese journal of ophthalmology · 2025Article
- Association of Weather Variables With Pathogens Contributing to Conjunctivitis Worldwide.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2025Article
- Article
- CD46 Is a Protein Receptor for Human Adenovirus Type 64.Viruses · 2024Article
- Alpha-defensin binding expands human adenovirus tropism.PLoS pathogens · 2024Article
- Etiological Study of Acute Conjunctivitis Caused by Human Adenovirus in Shanxi Province, China, between 2016 and 2019.Microbiology spectrum · 2023Article
- Cyclopentenylcytosine (CPE-C): In Vitro and In Vivo Evaluation as an Antiviral against Adenoviral Ocular Infections.Molecules (Basel, Switzerland) · 2023Article
- Broad sialic acid usage amongst species D human adenovirus.Npj viruses · 2023Article
- Ranpirnase (OKG-0301), a Novel Ribonuclease, Demonstrates Antiviral Activity against Adenovirus in the Ad5/NZW Rabbit Ocular Replication Model.Pathogens (Basel, Switzerland) · 2022Article
- Machine Learning Prediction of Adenovirus D8 Conjunctivitis Complications from Viral Whole-Genome Sequence.Ophthalmology science · 2022Article
- Molecular Epidemiology of Adenoviral Keratoconjunctivitis in Korea.Annals of laboratory medicine · 2022Article
- Evolution and diversity of human adenoviruses isolated from patients with keratoconjunctivitis.Iranian journal of microbiology · 2022Article
- Article
- Molecular Typing and Rapid Identification of Human Adenoviruses Associated With Respiratory Diseases Using Universal PCR and Sequencing Primers for the Three Major Capsid Genes: Penton Base, Hexon, and Fiber.Frontiers in microbiology · 2022Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
purposeHuman adenovirus species D type 19 (HAdV-D19) has been associated with epidemic keratoconjunctivitis (EKC), a highly inflammatory infection of the ocular surface. Confusion exists regarding the origins of HAdV-D19. The prototype virus (HAdV-D19p) does not cause EKC, while a virus identified later with the identical serologic determinant is a significant ocular pathogen.
methodsHigh throughput genome sequencing and bioinformatics analysis were performed on HAdV-D19p and three HAdV-D19 EKC strains, and compared to the previously sequenced clinical isolate, HAdV-D19 (C) and HAdV-D37. Corneas of C57BL/6J mice were injected with HAdV-D19p, HAdV-D19 (C), or virus-free buffer, and inflammation assessed by clinical examination, flow cytometry, and cytokine ELISA. Confocal microscopy and real-time PCR of infected corneal cell cultures were used to test viral entry.
resultsHAdV-D19 (C) and the other clinical EKC isolates showed nearly 100% sequence identity. EKC strains diverged from HAdV-D19p in the penton base, E3, and fiber transcription units. Simplot analysis showed recombination between EKC-associated HAdV-D19 with HAdV-D37, HAdV-D22, and HAdV-D19p, the latter contributing only the hexon gene, the principal serum neutralization determinant. HAdV-D19p induced stromal keratitis in the C57BL/6J mouse, but failed to infect productively human corneal epithelial cells. These data led to retyping of the clinical EKC isolates with a HAdV-D19 hexon gene as HAdV-D64.
conclusionsHAdV-D19 associated with EKC (HAdV-D64) originated from a recombination between HAdV-D19p, HAdV-D37, and HAdV-D22, and was mischaracterized because of a shared hexon gene. HAdV-D19p is not infectious for corneal epithelial cells, thus explaining the lack of any association with keratitis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.