ArticleApplied and environmental microbiology2012
Transcriptional and enzymatic profiling of Pleurotus ostreatus laccase genes in submerged and solid-state fermentation cultures.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 88 citations in OpenAlex.
- Asymmetric mitonuclear interactions trigger transgressive inheritance and mitochondria-dependent heterosis in hybrids of the model systemIMA fungus · 2025Article
- Expression profiling of laccase and β-glucan synthase genes in Pleurotus ostreatus during different developmental stages.Molecular biology reports · 2023Article
- Silencing of the Laccase (International journal of molecular sciences · 2023Article
- Laccase Production fromMicroorganisms · 2023Article
- Functional Characterization of Laccase Isozyme (PoLcc1) from the Edible MushroomInternational journal of molecular sciences · 2022Article
- Effect of Nutritional Factors and Copper on the Regulation of Laccase Enzyme Production inJournal of fungi (Basel, Switzerland) · 2021Article
- Strain Degeneration inJournal of fungi (Basel, Switzerland) · 2021Article
- Trends and Applications of Omics Technologies to Functional Characterisation of Enzymes and Protein Metabolites Produced by Fungi.Journal of fungi (Basel, Switzerland) · 2021Review
- An insight into transcriptome of Cyathus bulleri for lignocellulase expression on wheat bran.Archives of microbiology · 2021Article
- A Multiomic Approach to Understand HowJournal of fungi (Basel, Switzerland) · 2021Article
- Alternative Splicing of Heat Shock Transcription Factor 2 Regulates the Expression of Laccase Gene Family in Response to Copper inApplied and environmental microbiology · 2021Article
- Discovery of two novel laccase-like multicopper oxidases from Pleurotus citrinopileatus and their application in phenolic oligomer synthesis.Biotechnology for biofuels · 2021Article
- Laccase isoform diversity on basal medium in Cyathus bulleri and role in decolorization/detoxification of textile dyes and effluent.World journal of microbiology & biotechnology · 2020Article
- Glucose counteracts wood-dependent induction of lignocellulolytic enzyme secretion in monokaryon and dikaryon submerged cultures of the white-rot basidiomycete Pleurotus ostreatus.Scientific reports · 2020Article
- Crosslinked Enzyme Aggregates (CLEAs) of Laccases fromMolecules (Basel, Switzerland) · 2020Article
- Transcription Factor Atf1 Regulates Expression of Cellulase and Xylanase Genes during Solid-State Fermentation of Ascomycetes.Applied and environmental microbiology · 2019Article
- Molecular characterization and overexpression ofBiology open · 2019Article
- Differential transcriptomic profiling of filamentous fungus during solid-state and submerged fermentation and identification of an essential regulatory geneBiotechnology for biofuels · 2019Article
- Systematic Analysis of the Pleurotus ostreatus Laccase Gene (PoLac) Family and Functional Characterization of PoLac2 Involved in the Degradation of Cotton-Straw Lignin.Molecules (Basel, Switzerland) · 2018Article
- Integrative visual omics of the white-rot fungusBiotechnology for biofuels · 2018Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genome of the white rot basidiomycete Pleurotus ostreatus includes 12 phenol oxidase (laccase) genes. In this study, we examined their expression profiles in different fungal strains under different culture conditions (submerged and solid cultures) and in the presence of a wheat straw extract, which was used as an inducer of the laccase gene family. We used a reverse transcription-quantitative PCR (RT-qPCR)-based approach and focused on determining the reaction parameters (in particular, the reference gene set for the normalization and reaction efficiency determinations) used to achieve an accurate estimation of the relative gene expression values. The results suggested that (i) laccase gene transcription is upregulated in the induced submerged fermentation (iSmF) cultures but downregulated in the solid fermentation (SSF) cultures, (ii) the Lacc2 and Lacc10 genes are the main sources of laccase activity in the iSmF cultures upon induction with water-soluble wheat straw extracts, and (iii) an additional, as-yet-uncharacterized activity (Unk1) is specifically induced in SSF cultures that complements the activity of Lacc2 and Lacc10. Moreover, both the enzymatic laccase activities and the Lacc gene family transcription profiles greatly differ between closely related strains. These differences can be targeted for biotechnological breeding programs for enzyme production in submerged fermentation reactors.
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