ArticleJournal of Zhejiang University. Science. B2012
Extraction of DNA suitable for PCR applications from mature leaves of Mangifera indica L.
Article in Journal of Zhejiang University. Science. B, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 87 citations in OpenAlex.
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- Age and anatomical site shape the gastrointestinal bacterial communities of captive American crocodiles (Crocodylus acutus).Folia microbiologica · 2025Article
- Exploring Genetic Diversity and Inter-/Intraspecific Polymorphism inInternational journal of molecular sciences · 2025Article
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- Investigating the Effect of Bacilli and Lactic Acid Bacteria on Water Quality, Growth, Survival, Immune Response, and Intestinal Microbiota of CulturedAnimals : an open access journal from MDPI · 2024Article
- Coagulation of Hydrophobic Ionic Associates of Cetyltrimethylammonium Bromide and Carrageenan.Molecules (Basel, Switzerland) · 2023Article
- Use of polypyrrole-polystyrene membranes for extracting DNA from plant tissues.Biomaterials and biosystems · 2022Article
- Extraordinary Multi-Organismal Interactions Involving Bacteriophages, Bacteria, Fungi, and Rotifers: Quadruple Microbial Trophic Network in Water Droplets.International journal of molecular sciences · 2021Article
- Hybridization and introgression between Helicoverpa armigera and H. zea: an adaptational bridge.BMC evolutionary biology · 2020Article
- Chemical, antimicrobial, and molecular characterization of mortiño (Food science & nutrition · 2018Article
- DNA extraction protocol for biological ingredient analysis of Liuwei Dihuang Wan.Genomics, proteomics & bioinformatics · 2014Article
- Identification and characterization of plasma membrane aquaporins isolated from fiber cells of Calotropis procera.Journal of Zhejiang University. Science. B · 2013Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Good quality deoxyribonucleic acid (DNA) is the pre-requisite for its downstream applications. The presence of high concentrations of polysaccharides, polyphenols, proteins, and other secondary metabolites in mango leaves poses problem in getting good quality DNA fit for polymerase chain reaction (PCR) applications. The problem is exacerbated when DNA is extracted from mature mango leaves. A reliable and modified protocol based on the cetyltrimethylammonium bromide (CTAB) method for DNA extraction from mature mango leaves is described here. High concentrations of inert salt were used to remove polysaccharides; Polyvinylpyrrolidone (PVP) and β-mercaptoethanol were employed to manage phenolic compounds. Extended chloroform-isoamyl alcohol treatment followed by RNase treatment yielded 950-1050 µg of good quality DNA, free of protein and RNA. The problems of DNA degradation, contamination, and low yield due to irreversible binding of phenolic compounds and coprecipitation of polysaccharides with DNA were avoided by this method. The DNA isolated by the modified method showed good PCR amplification using simple sequence repeat (SSR) primers. This modified protocol can also be used to extract DNA from other woody plants having similar problems.
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Registered trials
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