ArticleDifferentiation; research in biological diversity2012
All-trans-retinoid acid induces the differentiation of encapsulated mouse embryonic stem cells into GABAergic neurons.
Article in Differentiation; research in biological diversity, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 36 citations in OpenAlex.
- The regulatory architecture of the primed pluripotent cell state.Nature communications · 2025Article
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- An alginate-based encapsulation system for delivery of therapeutic cells to the CNS.RSC advances · 2022Article
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- Generation of hiPSC-Derived Functional Dopaminergic Neurons in Alginate-Based 3D Culture.Frontiers in cell and developmental biology · 2021Article
- Activity of the human immortalized endothelial progenitor cell line HEPC-CB.1 supporting in vitro angiogenesis.Molecular biology reports · 2020Article
- A Fiber Alginate Co-culture Platform for the Differentiation of mESC and Modeling of the Neural Tube.Frontiers in neuroscience · 2020Article
- Comparative Evaluation of Hormones and Hormone-Like Molecule in Lineage Specification of Human Induced Pluripotent Stem Cells.International journal of stem cells · 2019Article
- Optogenetic control of iPS cell-derived neurons in 2D and 3D culture systems using channelrhodopsin-2 expression driven by the synapsin-1 and calcium-calmodulin kinase II promoters.Journal of tissue engineering and regenerative medicine · 2019Article
- Roles of Retinoic Acid Signaling in Shaping the Neuronal Architecture of the Developing Amphioxus Nervous System.Molecular neurobiology · 2018Article
- Article
- Distinct retinoic acid receptor (RAR) isotypes control differentiation of embryonal carcinoma cells to dopaminergic or striatopallidal medium spiny neurons.Scientific reports · 2017Article
- MicroRNA-125b-2 overexpression represses ectodermal differentiation of mouse embryonic stem cells.International journal of molecular medicine · 2015Article
- Functionalized α-Helical Peptide Hydrogels for Neural Tissue Engineering.ACS biomaterials science & engineering · 2015Article
- In vitro neurogenesis: development and functional implications of iPSC technology.Cellular and molecular life sciences : CMLS · 2014Review
- Retinoid signaling in control of progenitor cell differentiation during mouse development.Seminars in cell & developmental biology · 2013Review
- Effect of the environmental pollutant hexachlorobenzene (HCB) on the neuronal differentiation of mouse embryonic stem cells.International journal of environmental research and public health · 2013Article
- Comparative neuronal differentiation of self-renewing neural progenitor cell lines obtained from human induced pluripotent stem cells.Frontiers in cellular neuroscience · 2013Article
- A new multistep induction protocol for the transdifferentiation of bone marrow stromal stem cells into GABAergic neuron-like cells.Iranian biomedical journal · 2013Article
- RARβ2-dependent signaling represses neuronal differentiation in mouse ES cells.Differentiation; research in biological diversityArticle
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6 authors at 3 institutions in 1 country.
Funding
Abstract
Embryonic stem (ES) cells are pluripotent cells that can differentiate into all three main germ layers: endoderm, mesoderm, and ectoderm. Although a number of methods have been developed to differentiate ES cells into neuronal phenotypes such as sensory and motor neurons, the efficient generation of GABAergic interneurons from ES cells still presents an ongoing challenge. Because the main output of inhibitory GABAergic interneurons is the gamma-aminobutyric-acid (GABA), a neurotransmitter whose controlled homeostasis is required for normal brain function, the efficient generation in culture of functional interneurons may have future implications on the treatment of neurological disorders such as epilepsy, autism, and schizophrenia. The goal of this work was to examine the generation of GABAergic neurons from mouse ES cells by comparing an embryoid body-based methodology versus a hydrogel-based encapsulation protocol that involves the use of all-trans-retinoid acid (RA). We observed that (1) there was a 2-fold increase in neuronal differentiation in encapsulated versus non-encapsulated cells and (2) there was an increase in the specificity for interneuronal differentiation in encapsulated cells, as assessed by mRNA expression and electrophysiology approaches. Furthermore, our results indicate that most of the neurons obtained from encapsulated mouse ES cells are GABA-positive (∼87%). Thus, these results suggest that combining encapsulation of ES cells and RA treatment provide a more efficient and scalable differentiation strategy for the generation in culture of functional GABAergic interneurons. This technology may have implications for future cell replacement therapies and the treatment of CNS disorders.
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