ArticleMethods (San Diego, Calif.)2013
Directed evolution as a powerful synthetic biology tool.
Article in Methods (San Diego, Calif.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed.
- Synthetic Mirror Bacteria as a Frontier for Chiral Synthetic Biology and Biocontainment.MicrobiologyOpen · 2026Review
- Harnessing Nature's Algorithm: From Test Tubes to Autonomous In Vivo Evolution.Biotechnology journal · 2026Review
- Metabolism-based artificial organelles: From precise construction to smart theranostics.Materials today. Bio · 2025Review
- Molecular approaches for enhancing fermented bamboo-derived feed additives: A sustainable nutritional innovation for poultry.Poultry science · 2025Review
- Orthogonal RNA replication enables directed evolution and Darwinian adaptation in mammalian cells.Nature chemical biology · 2025Article
- Genetically encoded biosensors for the circular plastics bioeconomy.Metabolic engineering communications · 2024Article
- Engineering biology and automation-Replicability as a design principle.Engineering biology · 2024Review
- Engineering a Novel Probiotic Toolkit inACS synthetic biology · 2024Article
- Structural and mechanistic insights into Quinolone Synthase to address its functional promiscuity.Communications biology · 2024Article
- Machine learning model of the catalytic efficiency and substrate specificity of acyl-ACP thioesterase variants generated from natural andFrontiers in bioengineering and biotechnology · 2024Article
- Effective use of biosensors for high-throughput library screening for metabolite production.Journal of industrial microbiology & biotechnology · 2021Review
- Accelerating strain phenotyping with desorption electrospray ionization-imaging mass spectrometry and untargeted analysis of intact microbial colonies.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Development of a yeast cell surface display method using the SpyTag/SpyCatcher system.Scientific reports · 2021Article
- Controllable stereoinversion in DNA-catalyzed olefin cyclopropanationChemical science · 2021Article
- Rational Design of Adenylate Kinase Thermostability through Coevolution and Sequence Divergence Analysis.International journal of molecular sciences · 2021Article
- The Selection of Gamma-Ray Irradiated Higher Yield Rice Mutants by Directed Evolution Method.Plants (Basel, Switzerland) · 2020Article
- Beyond natural: synthetic expansions of botanical form and function.The New phytologist · 2020Review
- Review
- Generation of Cry11 Variants ofEvolutionary bioinformatics online · 2020Article
- Hydrogel Microparticles Functionalized with EngineeredSensors (Basel, Switzerland) · 2019Article
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3 authors.
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Abstract
At the heart of synthetic biology lies the goal of rationally engineering a complete biological system to achieve a specific objective, such as bioremediation and synthesis of a valuable drug, chemical, or biofuel molecule. However, the inherent complexity of natural biological systems has heretofore precluded generalized application of this approach. Directed evolution, a process which mimics Darwinian selection on a laboratory scale, has allowed significant strides to be made in the field of synthetic biology by allowing rapid identification of desired properties from large libraries of variants. Improvement in biocatalyst activity and stability, engineering of biosynthetic pathways, tuning of functional regulatory systems and logic circuits, and development of desired complex phenotypes in industrial host organisms have all been achieved by way of directed evolution. Here, we review recent contributions of directed evolution to synthetic biology at the protein, pathway, network, and whole cell levels.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.