ArticleBiochimica et biophysica acta2012
Inter-monomer electron transfer is too slow to compete with monomeric turnover in bc(1) complex.
Article in Biochimica et biophysica acta, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 24 citations in OpenAlex.
- Catalytic Reactions and Energy Conservation in the CytochromeChemical reviews · 2021Review
- Analysis of a Functional Dimer Model of Ubiquinol Cytochrome c Oxidoreductase.Biophysical journal · 2017Article
- pH and Potential Transients of the bcThe journal of physical chemistry. B · 2017Article
- Hybrid fusions show that inter-monomer electron transfer robustly supports cytochrome bc1 function in vivo.Biochemical and biophysical research communications · 2014Article
- A robust genetic system for producing heterodimeric native and mutant cytochrome bc(1).Biochemistry · 2013Article
- Analysis of the kinetics and bistability of ubiquinol:cytochrome c oxidoreductase.Biophysical journal · 2013Article
- The role of mitochondrial function and cellular bioenergetics in ageing and disease.The British journal of dermatology · 2013Review
- Catalytically-relevant electron transfer between two hemes bL in the hybrid cytochrome bc1-like complex containing a fusion of Rhodobacter sphaeroides and capsulatus cytochromes b.Biochimica et biophysica acta · 2013Article
- Role of the -PEWY-glutamate in catalysis at the Q(o)-site of the Cyt bc(1) complex.Biochimica et biophysica acta · 2013Article
- Design and use of photoactive ruthenium complexes to study electron transfer within cytochrome bc1 and from cytochrome bc1 to cytochrome c.Biochimica et biophysica actaReview
- The mechanism of ubihydroquinone oxidation at the Qo-site of the cytochrome bc1 complex.Biochimica et biophysica actaReview
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The homodimeric bc(1) complexes are membrane proteins essential in respiration and photosynthesis. The ~11Å distance between the two b(L)-hemes of the dimer opens the possibility of electron transfer between them, but contradictory reports make such inter-monomer electron transfer controversial. We have constructed in Rhodobacter sphaeroides a heterodimeric expression system similar to those used before, in which the bc(1) complex can be mutated differentially in the two copies of cyt b to test for inter-monomer electron transfer, but found that genetic recombination by cross-over then occurs to produce wild-type homodimer. Selection pressure under photosynthetic growth always favored the homodimer over heterodimeric variants enforcing inter-monomer electron transfer, showing that the latter are not competitive. These results, together with kinetic analysis of myxothiazol titrations, demonstrate that inter-monomer electron transfer does not occur at rates competitive with monomeric turnover. We examine the results from other groups interpreted as demonstrating rapid inter-monomer electron transfer, conclude that similar mechanisms are likely to be in play, and suggest that such claims might need to be re-examined.
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Registered trials
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