ArticleCurrent biology : CB2012
Uridylation of miRNAs by hen1 suppressor1 in Arabidopsis.
Article in Current biology : CB, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 92 papers.
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Who cites it
92 citing papers in PubMed, 168 citations in OpenAlex.
- Regulation of Pre-rRNA Processing in Plant: Mechanisms, Plasticity, and Developmental Implications.Plants (Basel, Switzerland) · 2026Review
- RNA Tailing by Nucleotidyltransferases in Plants: Mechanisms, Functions, and Biological Significance.Plants (Basel, Switzerland) · 2026Review
- Unveiling the wheat NTP gene family: insights from genome-wide identification and TaNTP6 haplotype analysis.BMC plant biology · 2025Article
- The action of Arabidopsis DICER-LIKE 2 in plant growth inhibition.The Plant cell · 2025Article
- Comprehensive Analysis of Small RNA Modifications inMetabolites · 2025Article
- HC-Pro inhibits HEN1 methyltransferase activity, leading to autophagic degradation of AGO1.Nature communications · 2025Article
- The Use of Arabidopsis thaliana to Characterize the Production and Action Stages of the Plant MicroRNA Pathway.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Scalable Approach to Evaluate Plant microRNA Trimming and Tailing from Small RNA-Seq Data.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Poly(U) polymerase activity in Caenorhabditis elegans regulates abundance and tailing of sRNA and mRNA.Genetics · 2024Article
- PBOX-sRNA-seq uncovers novel features of miRNA modification and identifies selected 5'-tRNA fragments bearing 2'-O-modification.Nucleic acids research · 2024Article
- Functions and mechanisms of RNA tailing by nucleotidyl transferase proteins in plants.Frontiers in plant science · 2024Review
- An extensive survey of phytoviral RNA 3' uridylation identifies extreme variations and virus-specific patterns.Plant physiology · 2023Article
- The expanding role of RNA modifications in plant RNA polymerase II transcripts: highlights and perspectives.Journal of experimental botany · 2023Review
- Small RNA modifications: regulatory molecules and potential applications.Journal of hematology & oncology · 2023Review
- Recent Insights into Plant miRNA Biogenesis: Multiple Layers of miRNA Level Regulation.Plants (Basel, Switzerland) · 2023Review
- Understanding the evolution of miRNA biogenesis machinery in plants with special focus on rice.Functional & integrative genomics · 2023Article
- microRNA production inFrontiers in plant science · 2023Review
- MicroRNA turnover: a tale of tailing, trimming, and targets.Trends in biochemical sciences · 2023Review
- Molecular mechanism underlying the di-uridylation activity of Arabidopsis TUTase URT1.Nucleic acids research · 2022Article
- Uridylation and the SKI complex orchestrate the Calvin cycle of photosynthesis through RNA surveillance ofProceedings of the National Academy of Sciences of the United States of America · 2022Article
32 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
HEN1-mediated 2'-O-methylation has been shown to be a key mechanism to protect plant microRNAs (miRNAs) and small interfering RNAs (siRNAs) as well as animal piwi-interacting RNAs (piRNAs) from degradation and 3' terminal uridylation [1-8]. However, enzymes uridylating unmethylated miRNAs, siRNAs, or piRNAs in hen1 are unknown. In this study, a genetic screen identified a second-site mutation hen1 suppressor1-2 (heso1-2) that partially suppresses the morphological phenotypes of the hypomorphic hen1-2 allele and the null hen1-1 allele in Arabidopsis. HESO1 encodes a terminal nucleotidyl transferase that prefers to add untemplated uridine to the 3' end of RNA, which is completely abolished by 2'-O-methylation. heso1-2 affects the profile of u-tailed miRNAs and siRNAs and increases the abundance of truncated and/or normal sized ones in hen1, which often results in increased total amount of miRNAs and siRNAs in hen1. In contrast, overexpressing HESO1 in hen1-2 causes more severe morphological defects and less accumulation of miRNAs. These results demonstrate that HESO1 is an enzyme uridylating unmethylated miRNAs and siRNAs in hen1. These observations also suggest that uridylation may destabilize unmethylated miRNAs through an unknown mechanism and compete with 3'-to-5' exoribonuclease activities in hen1. This study shall have implications on piRNA uridylation in hen1 in animals.
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