ArticleFrontiers in neural circuits2012
Two-photon scanning microscopy of in vivo sensory responses of cortical neurons genetically encoded with a fluorescent voltage sensor in rat.
Article in Frontiers in neural circuits, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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Who cites it
16 citing papers in PubMed, 19 citations in OpenAlex.
- Noise Sources and Strategies for Signal Quality Improvement in Biological Imaging: A Review Focused on Calcium and Cell Membrane Voltage Imaging.Biosensors · 2026Review
- Imaging different cell populations in the mouse olfactory bulb using the genetically encoded voltage indicator ArcLight.Neurophotonics · 2024Review
- Spatio-temporal parameters for optical probing of neuronal activity.Biophysical reviews · 2021Review
- Subcellular resolution three-dimensional light-field imaging with genetically encoded voltage indicators.Neurophotonics · 2020Article
- Corrigendum: Single-Neuron Level One-Photon Voltage Imaging With Sparsely Targeted Genetically Encoded Voltage Indicators.Frontiers in cellular neuroscience · 2019Article
- Single-Neuron Level One-Photon Voltage Imaging With Sparsely Targeted Genetically Encoded Voltage Indicators.Frontiers in cellular neuroscience · 2019Article
- Article
- Mammalian cortical voltage imaging using genetically encoded voltage indicators: a review honoring professor Amiram Grinvald.Neurophotonics · 2017Review
- Genetically Encoded Voltage Indicators: Opportunities and Challenges.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Review
- Designs and sensing mechanisms of genetically encoded fluorescent voltage indicators.Current opinion in chemical biology · 2015Review
- Monitoring brain activity with protein voltage and calcium sensors.Scientific reports · 2015Article
- Two-photon excitation microscopy and its applications in neuroscience.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Monitoring activity in neural circuits with genetically encoded indicators.Frontiers in molecular neuroscience · 2014Review
- Genetically encoded voltage sensor goes live.Nature biotechnology · 2013Article
- Probing neuronal activities with genetically encoded optical indicators: from a historical to a forward-looking perspective.Pflugers Archiv : European journal of physiology · 2013Review
- Two-photon voltage imaging using a genetically encoded voltage indicator.Scientific reports · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
A fluorescent voltage sensor protein "Flare" was created from a Kv1.4 potassium channel with YFP situated to report voltage-induced conformational changes in vivo. The RNA virus Sindbis introduced Flare into neurons in the binocular region of visual cortex in rat. Injection sites were selected based on intrinsic optical imaging. Expression of Flare occurred in the cell bodies and dendritic processes. Neurons imaged in vivo using two-photon scanning microscopy typically revealed the soma best, discernable against the background labeling of the neuropil. Somatic fluorescence changes were correlated with flashed visual stimuli; however, averaging was essential to observe these changes. This study demonstrates that the genetic modification of single neurons to express a fluorescent voltage sensor can be used to assess neuronal activity in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.