ArticleJournal of virological methods2012
A rapid label-free method for quantitation of human immunodeficiency virus type-1 particles by nanospectroscopy.
Article in Journal of virological methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 26 citations in OpenAlex.
- Analysis of Sigma-1 Receptor Antagonist BD1047 Effect on Upregulating Proteins in HIV-1-Infected Macrophages Exposed to Cocaine Using Quantitative Proteomics.Biomedicines · 2024Article
- High Stability and Low Power Nanometric Bio-Objects Trapping through Dielectric-Plasmonic Hybrid Nanobowtie.Biosensors · 2024Article
- Highly sensitive label-free biosensor: graphene/CaFScientific reports · 2023Article
- Comparative Analysis of the Interaction of Silver Nanoparticles with Hexokinase fromInternational journal of nanomedicine · 2023Article
- Selective Isolation of Retroviruses from Extracellular Vesicles by Intact Virion Immunoprecipitation.Bio-protocol · 2018Article
- Electron-Beam-Lithographed Nanostructures as Reference Materials for Label-Free Scattered-Light Biosensing of Single Filoviruses.Sensors (Basel, Switzerland) · 2018Article
- Membrane Fluidity Sensing on the Single Virus Particle Level with Plasmonic Nanoparticle Transducers.ACS sensors · 2017Article
- Recognition-mediated particle detection under microfluidic flow with waveguide-coupled 2D photonic crystals: towards integrated photonic virus detectors.Lab on a chip · 2017Article
- Label-free detection and manipulation of single biological nanoparticles.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2016Review
- Using optical trap to measure the refractive index of a single animal virus in culture fluid with high precision.Biomedical optics express · 2016Article
- Analytical technologies for influenza virus-like particle candidate vaccines: challenges and emerging approaches.Virology journal · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Infection of cells with human immunodeficiency virus type-1 (HIV-1) results in the production of both infectious and non-infectious virions. At present, several assays are available for the quantitation of virus particles based on the presence of either viral capsid protein or nucleic acid. However, the ability to detect the total number of virus particles, both infectious and non-infectious, has been an elusive goal that would advance the study of virus assembly and egress. A rapid optical detection scheme for real-time label-free quantitation of HIV-1 virus particles was developed. Virions produced in cell cultures transfected transiently were evaluated with a nanospectroscopic assay. Quantitation with the optical detection scheme correlated with routine conventional assays. Nanospectroscopy can be used for the detection of both infectious and non-infectious, wild type and mutant strains of HIV-1 in solution at concentrations as low as 7×10(10)particles/ml, requiring volumes as small as 2 μl per assay, and in significantly less time than standard techniques. This assay provides a rapid, reliable system for quantifying virus particles in solution and could be applied to the study of viral particle production in cell culture.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.