ArticleMolecular plant pathology2012
The novel virulence-related gene nlxA in the lipopolysaccharide cluster of Xanthomonas citri ssp. citri is involved in the production of lipopolysaccharide and extracellular polysaccharide, motility, biofilm formation and stress resistance.
Article in Molecular plant pathology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 40 citations in OpenAlex.
- Review
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- A Fluorescent Reporter-Based Evaluation Assay for Antibacterial Components AgainstFrontiers in microbiology · 2022Article
- The transcriptome landscapes of citrus leaf in different developmental stages.Plant molecular biology · 2021Article
- Mechanistic insights into host adaptation, virulence and epidemiology of the phytopathogen Xanthomonas.FEMS microbiology reviews · 2020Review
- The Role ofPathogens (Basel, Switzerland) · 2019Review
- TheJournal of bacteriology · 2019Article
- Biological and transcriptomic studies reveal hfq is required for swimming, biofilm formation and stress response in Xanthomonas axonpodis pv. citri.BMC microbiology · 2019Article
- Transposons and pathogenicity inPeerJ · 2018Article
- Proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp. citri during Citrus sinensis infection.BMC microbiology · 2017Article
- Xanthomonas citri ssp. citri requires the outer membrane porin OprB for maximal virulence and biofilm formation.Molecular plant pathology · 2017Article
- Deciphering the regulon of a GntR family regulator via transcriptome and ChIP-exo analyses and its contribution to virulence in Xanthomonas citri.Molecular plant pathology · 2017Article
- The influence of a modified lipopolysaccharide O-antigen on the biosynthesis of xanthan in Xanthomonas campestris pv. campestris B100.BMC microbiology · 2016Article
- Positive selection is the main driving force for evolution of citrus canker-causing Xanthomonas.The ISME journal · 2015Article
- Genome Sequencing of Xanthomonas vasicola Pathovar vasculorum Reveals Variation in Plasmids and Genes Encoding Lipopolysaccharide Synthesis, Type-IV Pilus and Type-III Secretion Effectors.Pathogens (Basel, Switzerland) · 2014Article
- Alteration of the phenotypic and pathogenic patterns of Burkholderia pseudomallei that persist in a soil environment.The American journal of tropical medicine and hygiene · 2014Article
- Insights into xanthomonas axonopodis pv. citri biofilm through proteomics.BMC microbiology · 2013Article
- Requirement of the lipopolysaccharide O-chain biosynthesis gene wxocB for type III secretion and virulence of Xanthomonas oryzae pv. Oryzicola.Journal of bacteriology · 2013Article
- Architecture and gene repertoire of the flexible genome of the extreme acidophile Acidithiobacillus caldus.PloS one · 2013Article
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3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lipopolysaccharide (LPS) is an important virulence factor of Xanthomonas citri ssp. citri, the causative agent of citrus canker disease. In this research, a novel gene, designated as nlxA (novel LPS cluster gene of X. citri ssp. citri), in the LPS cluster of X. citri ssp. citri 306, was characterized. Our results indicate that nlxA is required for O-polysaccharide biosynthesis by encoding a putative rhamnosyltransferase. This is supported by several lines of evidence: (i) NlxA shares 40.14% identity with WsaF, which acts as a rhamnosyltransferase; (ii) sodium dodecylsulphate-polyacrylamide gel electrophoresis analysis showed that four bands of the O-antigen part of LPS were missing in the LPS production of the nlxA mutant; this is also consistent with a previous report that the O-antigen moiety of LPS of X. citri ssp. citri is composed of a rhamnose homo-oligosaccharide; (iii) mutation of nlxA resulted in a significant reduction in the resistance of X. citri ssp. citri to different stresses, including sodium dodecylsulphate, polymyxin B, H(2)O(2), phenol, CuSO(4) and ZnSO(4). In addition, our results indicate that nlxA plays an important role in extracellular polysaccharide production, biofilm formation, stress resistance, motility on semi-solid plates, virulence and in planta growth in the host plant grapefruit.
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