ArticleBMC complementary and alternative medicine2012
Modulation of apoptosis in human hepatocellular carcinoma (HepG2 cells) by a standardized herbal decoction of Nigella sativa seeds, Hemidesmus indicus roots and Smilax glabra rhizomes with anti- hepatocarcinogenic effects.
Article in BMC complementary and alternative medicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 57 citations in OpenAlex.
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- Compound Kushen injection inhibits EMT of gastric cancer cells via the PI3K/AKT pathway.World journal of surgical oncology · 2022Article
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- Compound Kushen Injection Protects Skin From Radiation InjuryFrontiers in pharmacology · 2021Article
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- Network Pharmacology-Based Study on the Molecular Biological Mechanism of Action for Compound Kushen Injection in Anti-Cancer Effect.Medical science monitor : international medical journal of experimental and clinical research · 2020Article
- Medicinal plants commonly used against cancer in traditional medicine formulae in Sri Lanka.Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society · 2019Review
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- Review on the Potential Therapeutic Roles ofJournal of pharmacopuncture · 2017Review
- A Study on Cytotoxic and Apoptotic Potential of a Triterpenoid Saponin (3-O-BioMed research international · 2017Article
- In Vitro Anticancer Effect of Gedunin on Human Teratocarcinomal (NTERA-2) Cancer Stem-Like Cells.BioMed research international · 2017Article
- Anti-hepatocarcinogenic and Anti-oxidant Effects of Mangrove PlantPharmacognosy magazine · 2017Article
- Chemical composition of Nigella sativa Linn: Part 2 Recent advances.Inflammopharmacology · 2016Review
- Modulation of expression of heat shock proteins and apoptosis by Flueggea leucopyrus (Willd) decoction in three breast cancer phenotypes.BMC complementary and alternative medicine · 2015Article
- Inhibitory Effects of Chemical Compounds Isolated from the Rhizome of Smilax glabra on Nitric Oxide and Tumor Necrosis Factor- α Production in Lipopolysaccharide-Induced RAW264.7 Cell.Evidence-based complementary and alternative medicine : eCAM · 2015Article
- Antioxidant and Anti-Inflammatory Activities of Phenolic-Enriched Extracts of Smilax glabra.Evidence-based complementary and alternative medicine : eCAM · 2014Article
- Micropropagation effect on the anti-carcinogenic activitiy of polyphenolics from Mexican oregano (Poliomintha glabrescens Gray) in human colon cancer cells HT-29.Plant foods for human nutrition (Dordrecht, Netherlands) · 2013Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundA standardized poly-herbal decoction of Nigella sativa seeds, Hemidesmus indicus roots and Smilax glabra rhizomes used traditionally in Sri Lanka for cancer therapy has been demonstrated previously, to have anti-hepatocarcinogenic potential. Cytotoxicity, antioxidant activity, anti-inflammatory activity, and up regulation of p53 and p21 activities are considered to be some of the possible mechanisms through which the above decoction may mediate its anti-hepatocarcinogenic action. The main aim of the present study was to determine whether apoptosis is also a major mechanism by which the decoction mediates its anti-hepatocarcinogenic action.
methodsEvaluation of apoptosis in HepG2 cells was carried out by (a) microscopic observations of cell morphology, (b) DNA fragmentation analysis, (c) activities of caspase 3 and 9, as well as by (d) analysis of the expression of pro-apoptotic (Bax) and anti-apoptotic (Bcl-2) proteins associated with cell death.
resultsThe results demonstrated that in HepG2 cells, the decoction can induce (a) DNA fragmentation and (b) characteristic morphological changes associated with apoptosis (nuclear condensation, membrane blebbing, nuclear fragmentation and apoptotic bodies). The decoction could also, in a time and dose dependent manner, up regulate the expression of the pro-apoptotic gene Bax and down regulate expression of anti-apoptotic Bcl-2 gene (as evident from RT-PCR analysis, immunohistochemistry and western blotting). Further, the decoction significantly (p < .001) enhanced the activities of caspase-3 and caspase-9 in a time and dose dependent manner.
conclusionsOverall findings provide confirmatory evidence to demonstrate that the decoction may mediate its reported anti-hepatocarcinogenic effect, at least in part, through modulation of apoptosis.
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