ArticlePloS one2012
Characterization of periplasmic protein BP26 epitopes of Brucella melitensis reacting with murine monoclonal and sheep antibodies.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 47 citations in OpenAlex.
- Development of serologic diagnostic test based on in silico predicted synthetic peptides for Brucella canis in dogs.PloS one · 2026Article
- Multiepitope fusion protein-based ELISA for enhanced brucellosis serodiagnosis.PLoS neglected tropical diseases · 2025Article
- Development and evaluation of multiepitope fusion proteins for serological diagnosis of animal brucellosis.Microbiology spectrum · 2025Article
- Follow-up of antibody changes in brucellosis patients in Gansu, China.Microbiology spectrum · 2025Article
- A subunit vaccine based onActa biochimica et biophysica Sinica · 2024Article
- Production and evaluation of anti-BP26 monoclonal antibodies for the serological detection of animal brucellosis.Frontiers in veterinary science · 2024Article
- Development and evaluation of the recombinant BP26 protein-based C-ELISA for human brucellosis diagnosis.Frontiers in microbiology · 2024Article
- Identification of Potential Novel B-Cell Epitopes of Capsid Protein VP2 in Senecavirus A.Microbiology spectrum · 2023Article
- Screening of linear B-cell epitopes and its proinflammatory activities ofFrontiers in cellular and infection microbiology · 2023Article
- Evaluation of the Combined Use of Major Outer Membrane Proteins in the Serodiagnosis of Brucellosis.Infection and drug resistance · 2022Article
- Evaluation of Reactivity of Monoclonal Antibodies Against Omp25 ofFrontiers in cellular and infection microbiology · 2020Article
- miR-146b-5p Plays a Critical Role in the Regulation of Autophagy in ∆perBioMed research international · 2020Article
- Binding of Brucella protein, Bp26, to select extracellular matrix molecules.BMC molecular and cell biology · 2019Article
- Article
- Detection ofAntimicrobial resistance and infection control · 2019Article
- Diagnostic potential ofCentral-European journal of immunology · 2018Article
- Characterization of novel Omp31 antigenic epitopes of Brucella melitensis by monoclonal antibodies.BMC microbiology · 2017Article
- Immunoreactivity evaluation of a new recombinant chimeric protein againstIranian journal of microbiology · 2016Article
- A novel multi-epitope recombined protein for diagnosis of human brucellosis.BMC infectious diseases · 2016Article
- Expression, purification and immunochemical characterization of recombinant OMP28 protein of Brucella species.Open veterinary journal · 2016Article
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
More than 35,000 new cases of human brucellosis were reported in 2010 by the Chinese Center for Disease Control and Prevention. An attenuated B. melitensis vaccine M5-90 is currently used for vaccination of sheep and goats in China. In the study, a periplasmic protein BP26 from M5-90 was characterized for its epitope reactivity with mouse monoclonal and sheep antibodies. A total of 29 monoclonal antibodies (mAbs) against recombinant BP26 (rBP26) were produced, which were tested for reactivity with a panel of BP26 peptides, three truncated rBP26 and native BP26 containing membrane protein extracts (NMP) of B. melitensis M5-90 in ELISA and Western-Blot. The linear, semi-conformational and conformational epitopes from native BP26 were identified. Two linear epitopes recognized by mAbs were revealed by 28 of 16mer overlapping peptides, which were accurately mapped as the core motif of amino acid residues ⁹³DRDLQTGGI¹⁰¹ (position 93 to 101) or residues ¹⁰⁴QPIYVYPD¹¹¹, respectively. The reactivity of linear epitope peptides, rBP26 and NMP was tested with 137 sheep sera by ELISAs, of which the two linear epitopes had 65-70% reactivity and NMP 90% consistent with the results of a combination of two standard serological tests. The results were helpful for evaluating the reactivity of BP26 antigen in M5-90.
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