ArticlePloS one2012
How morphological constraints affect axonal polarity in mouse neurons.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 40 citations in OpenAlex.
- Nanomagnetic Guidance Shapes the Structure-Function Relationship of Developing Cortical Networks.Nano letters · 2024Article
- Beyond a Transmission Cable-New Technologies to Reveal the Richness in Axonal Electrophysiology.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Review
- Neurons-on-a-Chip:Molecules and cells · 2022Review
- Lab-on-Chip Microsystems forFrontiers in bioengineering and biotechnology · 2022Review
- Understanding, engineering, and modulating the growth of neural networks: An interdisciplinary approach.Biophysics reviews · 2021Review
- Multi-curvature micropatterns unveil distinct calcium and mitochondrial dynamics in neuronal networks.Lab on a chip · 2021Article
- Thermoplasmonic neural chip platform for in situ manipulation of neuronal connections in vitro.Nature communications · 2020Article
- Axonal cytomechanics in neuronal development.Journal of biosciences · 2020Review
- Assembly and Connection of Micropatterned Single Neurons for Neuronal Network Formation.Micromachines · 2018Article
- Effects of Morphology Constraint on Electrophysiological Properties of Cortical Neurons.Scientific reports · 2016Article
- Live-Cell, Label-Free Identification of GABAergic and Non-GABAergic Neurons in Primary Cortical Cultures Using Micropatterned Surface.PloS one · 2016Article
- Combining microfluidics, optogenetics and calcium imaging to study neuronal communication in vitro.PloS one · 2015Article
- Wnt-signaling and planar cell polarity genes regulate axon guidance along the anteroposterior axis in C. elegans.Developmental neurobiology · 2014Review
- Simulating cortical development as a self constructing process: a novel multi-scale approach combining molecular and physical aspects.PLoS computational biology · 2013Article
- Article
- Differential neurite outgrowth is required for axon specification by cultured hippocampal neurons.Journal of neurochemistry · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neuronal differentiation is under the tight control of both biochemical and physical information arising from neighboring cells and micro-environment. Here we wished to assay how external geometrical constraints applied to the cell body and/or the neurites of hippocampal neurons may modulate axonal polarization in vitro. Through the use of a panel of non-specific poly-L-lysine micropatterns, we manipulated the neuronal shape. By applying geometrical constraints on the cell body we provided evidence that centrosome location was not predictive of axonal polarization but rather follows axonal fate. When the geometrical constraints were applied to the neurites trajectories we demonstrated that axonal specification was inhibited by curved lines. Altogether these results indicated that intrinsic mechanical tensions occur during neuritic growth and that maximal tension was developed by the axon and expressed on straight trajectories. The strong inhibitory effect of curved lines on axon specification was further demonstrated by their ability to prevent formation of multiple axons normally induced by cytochalasin or taxol treatments. Finally we provided evidence that microtubules were involved in the tension-mediated axonal polarization, acting as curvature sensors during neuronal differentiation. Thus, biomechanics coupled to physical constraints might be the first level of regulation during neuronal development, primary to biochemical and guidance regulations.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.