ArticleNucleic acids research2012
Fast production of homogeneous recombinant RNA--towards large-scale production of RNA.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 65 citations in OpenAlex.
- Strategies to Reduce Promoter-Independent Transcription of DNA Nanostructures and Strand Displacement Complexes.ACS synthetic biology · 2024Review
- Back to the Future of Metabolism-Advances in the Discovery and Characterization of Unknown Biocatalytic Functions and Pathways.Life (Basel, Switzerland) · 2024Review
- Mechanisms and research advances in mRNA antibody drug-mediated passive immunotherapy.Journal of translational medicine · 2023Review
- Probing the conformational changes ofFrontiers in molecular biosciences · 2023Article
- RNAi-Based Therapeutics and Novel RNA Bioengineering Technologies.The Journal of pharmacology and experimental therapeutics · 2023Review
- Modified Nucleotides for Chemical and Enzymatic Synthesis of Therapeutic RNA.Current medicinal chemistry · 2023Article
- Recent Advances in Novel Recombinant RNAs for Studying Post-transcriptional Gene Regulation in Drug Metabolism and Disposition.Current drug metabolism · 2023Review
- Biomolecule-Based Optical Metamaterials: Design and Applications.Biosensors · 2022Review
- Production of Recombinant RNA in Escherichia coli Using Eggplant Latent Viroid as a Scaffold.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Intron-assisted, viroid-based production of insecticidal circular double-stranded RNA inRNA biology · 2021Article
- Novel approaches for efficient in vivo fermentation production of noncoding RNAs.Applied microbiology and biotechnology · 2020Review
- Advances and challenges in studying noncoding RNA regulation of drug metabolism and development of RNA therapeutics.Biochemical pharmacology · 2019Review
- Bioengineering of a single long noncoding RNA molecule that carries multiple small RNAs.Applied microbiology and biotechnology · 2019Article
- RNA therapy: Are we using the right molecules?Pharmacology & therapeutics · 2019Review
- RNA: packaged and protected by VLPs.RSC advances · 2018Article
- In vivo production of RNA nanostructures via programmed folding of single-stranded RNAs.Nature communications · 2018Article
- A guide to large-scale RNA sample preparation.Analytical and bioanalytical chemistry · 2018Review
- Engineering nucleic acid structures for programmable molecular circuitry and intracellular biocomputation.Nature chemistry · 2017Review
- New insights for therapeutic recombinant human miRNAs heterologous production: Rhodovolum sulfidophilum vs Escherichia coli.Bioengineered · 2017Article
- Bioengineered non-coding RNA agent (BERA) in action.Bioengineered · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the past decades, RNA molecules have emerged as important players in numerous cellular processes. To understand these processes at the molecular and atomic level, large amounts of homogeneous RNA are required for structural, biochemical and pharmacological investigations. Such RNAs are generally obtained from laborious and costly in vitro transcriptions or chemical synthesis. In 2007, a recombinant RNA technology has been described for the constitutive production of large amounts of recombinant RNA in Escherichia coli using a tRNA-scaffold approach. We demonstrate a general applicable extension to the described approach by introducing the following improvements: (i) enhanced transcription of large recombinant RNAs by T7 RNA polymerase (high transcription rates, versatile), (ii) efficient and facile excision of the RNA of interest from the tRNA-scaffold by dual cis-acting hammerhead ribozyme mediated cleavage and (iii) rapid purification of the RNA of interest employing anion-exchange chromatography or affinity chromatography followed by denaturing polyacrylamide gel electrophoresis. These improvements in the existing method pave the tRNA-scaffold approach further such that any (non-)structured product RNA of a defined length can cost-efficiently be obtained in (multi-)milligram quantities without in vitro enzymatic manipulations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.