ArticleMolecular biology of the cell2012
Protein kinase C δ regulates the release of collagen type I from vascular smooth muscle cells via regulation of Cdc42.
Article in Molecular biology of the cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- Correlation of the serum cell division cycle 42 with CD4Frontiers in cardiovascular medicine · 2024Article
- The Role of Cdc42 in the Insulin and Leptin Pathways Contributing to the Development of Age-Related Obesity.Nutrients · 2023Review
- Article
- Articular Chondrocyte Phenotype Regulation through the Cytoskeleton and the Signaling Processes That Originate from or Converge on the Cytoskeleton: Towards a Novel Understanding of the Intersection between Actin Dynamics and Chondrogenic Function.International journal of molecular sciences · 2021Review
- Proinflammation, profibrosis, and arterial aging.Aging medicine (Milton (N.S.W)) · 2020Review
- Article
- Review
- The frontal skull Hounsfield unit value can predict ventricular enlargement in patients with subarachnoid haemorrhage.Scientific reports · 2018Article
- RhoA, Rac1, and Cdc42 differentially regulate αSMA and collagen I expression in mesenchymal stem cells.The Journal of biological chemistry · 2018Article
- Collagen I: a kingpin for rotator cuff tendon pathology.American journal of translational research · 2018Review
- Cells lay their own tracks - optogenetic Cdc42 activation stimulates fibronectin deposition supporting directed migration.Journal of cell science · 2017Article
- Protein Kinase C as Regulator of Vascular Smooth Muscle Function and Potential Target in Vascular Disorders.Advances in pharmacology (San Diego, Calif.) · 2017Review
- The Late Endosome and Its Lipid BMP Act as Gateways for Efficient Cytosolic Access of the Delivery Agent dfTAT and Its Macromolecular Cargos.Cell chemical biology · 2016Article
- Andrographolide Ameliorates Abdominal Aortic Aneurysm Progression by Inhibiting Inflammatory Cell Infiltration through Downregulation of Cytokine and Integrin Expression.The Journal of pharmacology and experimental therapeutics · 2016Article
- Targeting Rho-GTPases in immune cell migration and inflammation.British journal of pharmacology · 2014Review
- Protein kinase C-δ (PKCδ) regulates proinflammatory chemokine expression through cytosolic interaction with the NF-κB subunit p65 in vascular smooth muscle cells.The Journal of biological chemistry · 2014Article
- Trafficking mechanisms of extracellular matrix macromolecules: insights from vertebrate development and human diseases.The international journal of biochemistry & cell biology · 2014Review
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Collagen type I is the most abundant component of extracellular matrix in the arterial wall. Mice knocked out for the protein kinase C δ gene (PKCδ KO) show a marked reduction of collagen I in the arterial wall. The lack of PKCδ diminished the ability of arterial smooth muscle cells (SMCs) to secrete collagen I without significantly altering the intracellular collagen content. Moreover, the unsecreted collagen I molecules accumulate in large perinuclear puncta. These perinuclear structures colocalize with the trans-Golgi network (TGN) marker TGN38 and to a lesser degree with cis-Golgi marker (GM130) but not with early endosomal marker (EEA1). Associated with diminished collagen I secretion, PKCδ KO SMCs exhibit a significant reduction in levels of cell division cycle 42 (Cdc42) protein and mRNA. Restoring PKCδ expression partially rescues Cdc42 expression and collagen I secretion in PKCδ KO SMCs. Inhibition of Cdc42 expression or activity with small interfering RNA or secramine A in PKCδ WT SMCs eliminates collagen I secretion. Conversely, restoring Cdc42 expression in PKCδ KO SMCs enables collagen I secretion. Taken together, our data demonstrate that PKCδ mediates collagen I secretion from SMCs, likely through a Cdc42-dependent mechanism.
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Registered trials
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