ArticleMolecular biology of the cell2012
cPLA2α and EHD1 interact and regulate the vesiculation of cholesterol-rich, GPI-anchored, protein-containing endosomes.
Article in Molecular biology of the cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 47 citations in OpenAlex.
- A Quarter Century of EHD Protein Research: From Endosomal Recycling to Ciliopathies.Traffic (Copenhagen, Denmark) · 2026Review
- Endosomal actin attenuation and fission are regulated by MICAL2.Journal of cell science · 2026Article
- The endocytic fission protein EHD1 interacts with tubulin and regulates microtubule function.Biochimica et biophysica acta. Molecular cell research · 2026Article
- Investigation of Intercellular Trafficking of Global Glycosylphosphatidylinositol-Anchored Proteins Using Cell Metabolic Engineering and Transwell Coculture.Angewandte Chemie (International ed. in English) · 2026Article
- Endosomal actin branching, fission, and receptor recycling require FCHSD2 recruitment by MICAL-L1.Molecular biology of the cell · 2024Article
- Investigation of Glycosylphosphatidylinositol (GPI)-Plasma Membrane Interaction in Live Cells and the Influence of GPI Glycan Structure on the Interaction.Chemistry (Weinheim an der Bergstrasse, Germany) · 2024Article
- Advances and challenges in understanding endosomal sorting and fission.The FEBS journal · 2023Article
- Profiling Glycosylphosphatidylinositol (GPI)-Interacting Proteins in the Cell Membrane Using a Bifunctional GPI Analogue as the Probe.Journal of proteome research · 2023Article
- Coronin2A links actin-based endosomal processes to the EHD1 fission machinery.Molecular biology of the cell · 2022Article
- Raft platforms highly enriched in cholesterol: major scaffolds for IL-6 signalling assembly with implications in inflammation and cancer.The FEBS journal · 2022Article
- WIPI1 promotes fission of endosomal transport carriers and formation of autophagosomes through distinct mechanisms.Autophagy · 2021Article
- Chip-Based Sensing of the Intercellular Transfer of Cell Surface Proteins: Regulation by the Metabolic State.Biomedicines · 2021Article
- Cellular functions and intrinsic attributes of the ATP-binding Eps15 homology domain-containing proteins.Protein science : a publication of the Protein Society · 2020Review
- The Cytosolic Phospholipase ABiomolecules · 2020Article
- Sorting nexin 17 (SNX17) links endosomal sorting to Eps15 homology domain protein 1 (EHD1)-mediated fission machinery.The Journal of biological chemistry · 2020Article
- Article
- Structural basis of phosphatidylcholine recognition by the C2-domain of cytosolic phospholipase AeLife · 2019Article
- ATP-dependent membrane remodeling links EHD1 functions to endocytic recycling.Nature communications · 2018Article
- Tying trafficking to fusion and fission at the mighty mitochondria.Traffic (Copenhagen, Denmark) · 2018Review
- The enigmatic endosome - sorting the ins and outs of endocytic trafficking.Journal of cell science · 2018Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The lipid modifier phospholipase A2 catalyzes the hydrolysis of phospholipids to inverted-cone-shaped lysophospholipids that contribute to membrane curvature and/or tubulation. Conflicting findings exist regarding the function of cytosolic phospholipase A2 (cPLA2) and its role in membrane regulation at the Golgi and early endosomes. However, no studies addressed the role of cPLA2 in the regulation of cholesterol-rich membranes that contain glycosylphosphatidylinositol-anchored proteins (GPI-APs). Our studies support a role for cPLA2α in the vesiculation of GPI-AP-containing membranes, using endogenous CD59 as a model for GPI-APs. On cPLA2α depletion, CD59-containing endosomes became hypertubular. Moreover, accumulation of lysophospholipids induced by a lysophospholipid acyltransferase inhibitor extensively vesiculated CD59-containing endosomes. However, overexpression of cPLA2α did not increase the endosomal vesiculation, implying a requirement for additional factors. Indeed, depletion of the "pinchase" EHD1, a C-terminal Eps15 homology domain (EHD) ATPase, also induced hypertubulation of CD59-containing endosomes. Furthermore, EHD1 and cPLA2α demonstrated in situ proximity (<40 nm) and interacted in vivo. The results presented here provide evidence that the lipid modifier cPLA2α and EHD1 are involved in the vesiculation of CD59-containing endosomes. We speculate that cPLA2α induces membrane curvature and allows EHD1, possibly in the context of a complex, to sever the curved membranes into vesicles.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.