ArticleCell cycle (Georgetown, Tex.)2012
Chemical genetic induction of meiosis in Schizosaccharomyces pombe.
Article in Cell cycle (Georgetown, Tex.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- Mutual, spatially limited control of meiotic DNA break formation by Mre11-Rad50-Nbs1 DNA repair complex and Tel1 (ATM) protein kinase.Nucleic acids research · 2025Article
- A bifunctional snoRNA with separable activities in guiding rRNA 2'-O-methylation and scaffolding gametogenesis effectors.Nature communications · 2025Article
- Mug20-Rec25-Rec27 binds DNA and enhances meiotic DNA break formation via phase-separated condensates.Nucleic acids research · 2025Article
- Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.Scientific reports · 2024Article
- SILAC-Based Proteomic Analysis of Meiosis in the Fission Yeast Schizosaccharomyces pombe.Methods in molecular biology (Clifton, N.J.) · 2023Article
- S. pombe wtf drivers use dual transcriptional regulation and selective protein exclusion from spores to cause meiotic drive.PLoS genetics · 2022Article
- Dynamic configurations of meiotic DNA-break hotspot determinant proteins.Journal of cell science · 2022Article
- The Meiosis-Specific Crs1 Cyclin Is Required for Efficient S-Phase Progression and Stable Nuclear Architecture.International journal of molecular sciences · 2021Article
- Activation of meiotic recombination by nuclear import of the DNA break hotspot-determining complex in fission yeast.Journal of cell science · 2021Article
- Proteomic analysis of meiosis and characterization of novel short open reading frames in the fission yeastCell cycle (Georgetown, Tex.) · 2020Article
- Physical basis for long-distance communication along meiotic chromosomes.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Quantitative Genome-Wide Measurements of Meiotic DNA Double-Strand Breaks and Protein Binding in S. pombe.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Modulation of plant growth in vivo and identification of kinase substrates using an analog-sensitive variant of CYCLIN-DEPENDENT KINASE A;1.BMC plant biology · 2016Article
- Mal3, the Schizosaccharomyces pombe homolog of EB1, is required for karyogamy and for promoting oscillatory nuclear movement during meiosis.Cell cycle (Georgetown, Tex.) · 2014Article
- DNA intermediates of meiotic recombination in synchronous S. pombe at optimal temperature.Nucleic acids research · 2014Article
- Dissecting the first and the second meiotic divisions using a marker-less drug-hypersensitive fission yeast.Cell cycle (Georgetown, Tex.) · 2014Article
- Synchronized fission yeast meiosis using an ATP analog-sensitive Pat1 protein kinase.Nature protocols · 2014Article
- Protein kinases required for proper segregation of chromosomes during meiosis.Cell cycle (Georgetown, Tex.) · 2013Article
- A knockout screen for protein kinases required for the proper meiotic segregation of chromosomes in the fission yeast Schizosaccharomyces pombe.Cell cycle (Georgetown, Tex.) · 2013Article
- A new tool for an old problem: synchronizing fission yeast cells during meiosis using an ATP analog-sensitive protein kinase.Cell cycle (Georgetown, Tex.) · 2012Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the fission yeast Schizosaccharomyces pombe, meiosis is inhibited by the protein kinase Pat1, which phosphorylates and inactivates Mei2, an RNA binding protein essential for the initiation of meiosis. When diploid cells are deprived of nutrients, they initiate a cascade of events leading to the inactivation of Pat1 and entry into meiosis. Strains carrying the temperature-sensitive pat1-114 allele are forced to enter into meiosis when shifted to the non-permissive temperature, independently of the ploidity of the cell. This system has been extensively used, since it is possible to achieve a highly synchronous meiosis, which is a must for any molecular or microscopic approach that aims to decipher the mechanisms governing meiosis. Here, we have designed a new system to obtain a similarly synchronous meiosis, but independently of temperature shifts. Thus, by introducing a mutation in the ATP pocket of Pat1, we have generated a protein kinase that, in the presence of small specific inhibitors, can be inactivated. This results in forced entry into meiosis without the need of a temperature shift, minimizing the introduction of heat shock or any other stress responses along the meiotic waves of transcription.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.