ArticleGene therapy2013
Electroporation-mediated gene transfer directly to the swine heart.
Article in Gene therapy, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 45 citations in OpenAlex.
- Article
- Short microsecond pulses achieve homogeneous electroporation of elongated biological cells irrespective of their orientation in electric field.Scientific reports · 2020Article
- Gene therapy for atrial fibrillation - How close to clinical implementation?International journal of cardiology · 2019Review
- Gene Therapy for the Treatment of Cardiac Arrhythmias: Current and Emerging Applications.The Journal of innovations in cardiac rhythm management · 2018Review
- Gene Therapy Approaches to Biological Pacemakers.Journal of cardiovascular development and disease · 2018Review
- Development of a Mouse Model of Prostate Cancer Using the Sleeping Beauty Transposon and Electroporation.Molecules (Basel, Switzerland) · 2018Article
- Improvement in Electrotransfection of Cells Using Carbon-Based Electrodes.Cellular and molecular bioengineering · 2016Article
- Cardiac gene therapy: are we there yet?Gene therapy · 2016Review
- Vascular endothelial growth factor-A gene electrotransfer promotes angiogenesis in a porcine model of cardiac ischemia.Gene therapy · 2016Article
- Biological Therapies for Atrial Fibrillation: Ready for Prime Time?Journal of cardiovascular pharmacology · 2016Review
- A pliable electroporation patch (ep-Patch) for efficient delivery of nucleic acid molecules into animal tissues with irregular surface shapes.Scientific reports · 2015Article
- Article
- Electroporation-mediated gene delivery.Advances in genetics · 2015Article
- The Use of Gene Therapy for Ablation of Atrial Fibrillation.Arrhythmia & electrophysiology review · 2014Article
- Physical energy for drug delivery; poration, concentration and activation.Advanced drug delivery reviews · 2014Review
- Article
- Gene therapy delivery systems for enhancing viral and nonviral vectors for cardiac diseases: current concepts and future applications.Human gene therapy · 2013Review
- Cell-specific targeting strategies for electroporation-mediated gene delivery in cells and animals.The Journal of membrane biology · 2013Review
- Myocardial gene transfer: routes and devices for regulation of transgene expression by modulation of cellular permeability.Human gene therapy · 2013Review
- Plasmid IL-12 electroporation in melanoma.Human vaccines & immunotherapeutics · 2012Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
In vivo gene transfer to the ischemic heart via electroporation holds promise as a potential therapeutic approach for the treatment of heart disease. In the current study, we investigated the use of in vivo electroporation for gene transfer using three different penetrating electrodes and one non-penetrating electrode. The hearts of adult male swine were exposed through a sternotomy. Eight electric pulses synchronized to the rising phase of the R wave of the electrocardiogram were administered at varying pulse widths and field strengths following an injection of either a plasmid encoding luciferase or one encoding green fluorescent protein. Four sites on the anterior wall of the left ventricle were treated. Animals were killed 48 h after injection and electroporation and gene expression was determined. Results were compared with sites in the heart that received plasmid injection but no electric pulses or were not treated. Gene expression was higher in all electroporated sites when compared with injection only sites demonstrating the robustness of this approach. Our results provide evidence that in vivo electroporation can be a safe and effective non-viral method for delivering genes to the heart, in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.