ArticleStem cells and development2012
Germline transmission of a novel rat embryonic stem cell line derived from transgenic rats.
Article in Stem cells and development, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 24 citations in OpenAlex.
- 2015 Guidelines for Establishing Genetically Modified Rat Models for Cardiovascular Research.Journal of cardiovascular translational research · 2015Guideline
- Establishment and longitudinal characterisation of a feeder-free embryonic stem-like cell line (ATES1) derived from blastomeres of the climbing perch, Anabas testudineus.Fish physiology and biochemistry · 2026Article
- A Pilot Study of Bone Marrow Transplantation in a GALT-Null Rat Model of Classic Galactosemia.JIMD reports · 2025Article
- Rat Models of Hormone Receptor-Positive Breast Cancer.Journal of mammary gland biology and neoplasia · 2024Review
- Production of large, defined genome modifications in rats by targeting rat embryonic stem cells.Stem cell reports · 2023Article
- Rat Embryonic Stem Cell Transgenesis.Methods in molecular biology (Clifton, N.J.) · 2023Article
- A novel approach to differentiate rat embryonic stem cells in vitro reveals a role for RNF12 in activation of X chromosome inactivation.Scientific reports · 2019Article
- Gene Editing in Rat Embryonic Stem Cells to Produce In Vitro Models and In Vivo Reporters.Stem cell reports · 2017Article
- Rat Genome and Model Resources.ILAR journal · 2017Review
- Derivation of Transgene-Free Rat Induced Pluripotent Stem Cells Approximating the Quality of Embryonic Stem Cells.Stem cells translational medicine · 2017Article
- Efficient generation of selection-gene-free rat knockout models by homologous recombination in ES cells.FEBS letters · 2016Article
- Rat embryonic stem cells create new era in development of genetically manipulated rat models.World journal of stem cells · 2015Review
- CRISPR/Cas-mediated genome editing in the rat via direct injection of one-cell embryos.Nature protocols · 2014Article
- Generation and characterization of bat-induced pluripotent stem cells.Theriogenology · 2014Article
- Effect of Gsk3 inhibitor CHIR99021 on aneuploidy levels in rat embryonic stem cells.In vitro cellular & developmental biology. Animal · 2014Article
- Effect of leukemia inhibitory factor and forskolin on establishment of rat embryonic stem cell lines.The Journal of reproduction and development · 2014Article
- Histone modification of embryonic stem cells produced by somatic cell nuclear transfer and fertilized blastocysts.Cell journal · 2014Article
- Generation of naive-like porcine-induced pluripotent stem cells capable of contributing to embryonic and fetal development.Stem cells and development · 2013Article
- Article
- A Comparison of Automated Perfusion- and Manual Diffusion-Based Human Regulatory T Cell Expansion and Functionality Using a Soluble Activator Complex.Cell transplantationArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Germline-competent rat embryonic stem (ES) cell lines are important resources for the creation of mutant rat models using ES-cell-based gene targeting technology. The ability to isolate germline-competent ES cell lines from any rat strain, including genetically modified strains, would allow for more sophisticated genetic manipulations without extensive breeding. Sprague Dawley (SD) males carrying an enhanced green fluorescent protein (EGFP) transgene were used as the founder animals for the derivation of ES cell lines. A number of ES cell lines were established and subjected to rigorous quality control testing that included assessment of pluripotency factor expression, karyotype analysis, and pathogen/sterility testing. Two male ES cell lines, SD-Tg.EC1/Rrrc and SD-Tg.EC8/Rrrc, were injected into blastocysts recovered from a cross of Dark Agouti (DA) males with SD females. Resulting chimeric animals were bred with wild-type SD mates to verify the germline transmissibility of the ES cell lines by identifying pups carrying the ES cell line-derived EGFP transgene. While both ES cell lines gave rise to chimeric animals, only SD-Tg.EC1 was germline competent. This confirms the feasibility of deriving germline-competent ES cell lines from transgenic rat strains and provides a novel ES cell line with a stable green fluorescent protein (GFP) reporter for future genetic manipulations to create new rat models.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.