ArticleBiomacromolecules2012
Engineering a polymeric gene delivery vector based on poly(ethylenimine) and hyaluronic acid.
Article in Biomacromolecules, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 50 citations in OpenAlex.
- Synthesis of self-assembled hyaluronan based nanoparticles and their applications in targeted imaging and therapy.Carbohydrate research · 2022Review
- Novel zwitterionic vectors: Multi-functional delivery systems for therapeutic genes and drugs.Computational and structural biotechnology journal · 2020Review
- A mechanistic investigation exploring the differential transfection efficiencies between the easy-to-transfect SK-BR3 and difficult-to-transfect CT26 cell lines.Journal of nanobiotechnology · 2017Article
- Drug-Loaded Polymeric Nanoparticles for Cancer Stem Cell Targeting.Frontiers in pharmacology · 2017Review
- Systematically probing the bottom-up synthesis of AuPAMAM conjugates for enhanced transfection efficiency.Journal of nanobiotechnology · 2016Article
- Polysaccharide-based Noncovalent Assembly for Targeted Delivery of Taxol.Scientific reports · 2016Article
- When 1+1>2: Nanostructured composites for hard tissue engineering applications.Materials science & engineering. C, Materials for biological applications · 2015Review
- A simple and efficient method for transfecting mouse embryonic stem cells using polyethylenimine.Experimental cell research · 2015Article
- Targeted polymeric nanoparticles for cancer gene therapy.Journal of drug targeting · 2015Review
- Osteochondral tissue regeneration through polymeric delivery of DNA encoding for the SOX trio and RUNX2.Acta biomaterialia · 2014Article
- Gelatin carriers for drug and cell delivery in tissue engineering.Journal of controlled release : official journal of the Controlled Release Society · 2014Review
- Recent advances in live cell imaging of hepatoma cells.BMC cell biology · 2014Review
- Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity.Tissue engineering. Part C, Methods · 2014Article
- Hyaluronic acid conjugates as vectors for the active targeting of drugs, genes and nanocomposites in cancer treatment.Molecules (Basel, Switzerland) · 2014Review
- Optimization of PAMAM-gold nanoparticle conjugation for gene therapy.Biomaterials · 2014Article
- Modulation of polyplex release from biodegradable microparticles through poly(ethylenimine) modification and varying loading concentration.Pharmaceutical research · 2014Article
- Fabrication of core-shell nanoparticles via controlled aggregation of semi-flexible conjugated polymer and hyaluronic acid.Macromolecules · 2013Article
- Building bridges: leveraging interdisciplinary collaborations in the development of biomaterials to meet clinical needs.Advanced materials (Deerfield Beach, Fla.) · 2012Review
- Efficacy and mechanism of poloxamine-assisted polyplex transfection.The journal of gene medicineArticle
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
In this work, the effects of primary amines, ligand targeting, and overall charge on the effectiveness of branched poly(ethylenimine)-hyaluronic acid conjugate (bPEI-HA) zwitterionic gene delivery vectors are investigated. To elucidate the relative importance of each of these parameters, we explored the zeta potential, cytotoxicity, and transfection efficiency for a variety of formulations of bPEI-HA. It was found that the length of the hyaluronic acid (HA) oligosaccharide had the most significant effect on cytotoxicity and transfection efficiency with human mesenchymal stem cells. Test groups of bPEI incorporating HA with a length of 10 saccharides had significantly higher transfection efficiency (14.6 ± 2.0%) and lower cytotoxicity than other formulations tested, with the cytotoxicity of the group containing the greatest mass of 10 saccharide showing similar results as the positive controls at the highest polymer concentration (100 μg/mL). Additionally, molar incorporation of HA, as opposed to the saccharide length and HA mass incorporation, had the greatest effect on zeta potential but a minor effect on both cytotoxicity and transfection efficiency. This work demonstrates the relative importance of each of these tunable design criteria when creating a zwitterionic polymeric gene delivery vector and provides useful specific information regarding the design of bPEI-HA gene delivery vectors.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.