ArticleVeterinary research2012
Pan-genomic analysis of bovine monocyte-derived macrophage gene expression in response to in vitro infection with Mycobacterium avium subspecies paratuberculosis.
Article in Veterinary research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.
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Who cites it
37 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Systematic comparison of ranking aggregation methods for gene lists in experimental results.Bioinformatics (Oxford, England) · 2022Pooled it
- Evaluation of the potential virulence of Mycobacterium avium subspecies paratuberculosis isolates from cattle in Uganda.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Comparison of four culture protocols for differentiating bovine peripheral blood mononuclear cells into macrophages.Frontiers in veterinary science · 2026Article
- Weighted gene co-expression network analysis reveals key genes and lncRNAs inFrontiers in immunology · 2026Article
- Functional genomics of trypanotolerant and trypanosusceptible cattle infected with Trypanosoma congolense across multiple time points and tissues.PLoS neglected tropical diseases · 2025Article
- Article
- The role of interleukin-10 receptor alpha (IL10Rα) in Mycobacterium avium subsp. paratuberculosis infection of a mammary epithelial cell line.BMC genomic data · 2024Article
- In-depth systems biological evaluation of bovine alveolar macrophages suggests novel insights into molecular mechanisms underlyingFrontiers in microbiology · 2022Article
- A framework for non-preserved consensus gene module detection in Johne's disease.Frontiers in veterinary science · 2022Article
- Article
- The Impact of Whole Sesame Seeds on the Expression of Key-Genes Involved in the Innate Immunity of Dairy Goats.Animals : an open access journal from MDPI · 2021Article
- Integrated Analysis of lncRNAs, mRNAs, and TFs to Identify Regulatory Networks Underlying MAP Infection in Cattle.Frontiers in genetics · 2021Article
- Analysis of mRNA and circRNA Expression Profiles of Bovine Monocyte-Derived Macrophages Infected WithFrontiers in microbiology · 2021Article
- Fasciola hepatica products can alter the response of bovine immune cells to Mycobacterium avium subsp. paratuberculosis.Parasite immunology · 2020Article
- Modelling Bovine Granuloma Formation In Vitro upon Infection withVeterinary sciences · 2019Article
- Analysis of the microRNA Expression Profile of Bovine Monocyte-derived Macrophages Infected withInternational journal of molecular sciences · 2019Article
- Analysis of long non-coding RNA and mRNA expression in bovine macrophages brings up novel aspects of Mycobacterium avium subspecies paratuberculosis infections.Scientific reports · 2019Article
- Transcriptome Profiling of Bovine Macrophages Infected byFrontiers in immunology · 2019Article
- Alveolar macrophage functions during the transition phase to active immunity in calves1.Journal of animal science · 2018Article
- Pathways and Genes Associated with Immune Dysfunction in Sheep Paratuberculosis.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mycobacterium avium subspecies paratuberculosis is the causative agent of Johne's disease, an intestinal disease of ruminants with major economic consequences. Infectious bacilli are phagocytosed by host macrophages upon exposure where they persist, resulting in lengthy subclinical phases of infection that can lead to immunopathology and disease dissemination. Consequently, analysis of the macrophage transcriptome in response to M. avium subsp. paratuberculosis infection can provide valuable insights into the molecular mechanisms that underlie Johne's disease. Here, we investigate pan-genomic gene expression in bovine monocyte-derived macrophages (MDM) purified from seven age-matched females, in response to in vitro infection with M. avium subsp. paratuberculosis (multiplicity of infection 2:1) at intervals of 2 hours, 6 hours and 24 hours post-infection (hpi). Differentially expressed genes were identified by comparing the transcriptomes of the infected MDM to the non-infected control MDM at each time point (adjusted P-value threshold ≤ 0.10). 1050 differentially expressed unique genes were identified 2 hpi, with 974 and 78 differentially expressed unique genes detected 6 and 24 hpi, respectively. Furthermore, in the infected MDM the number of upregulated genes exceeded the number of downregulated genes at each time point, with the fold-change in expression for the upregulated genes markedly higher than that for the downregulated genes. Inspection and systems biology analysis of the differentially expressed genes revealed an enrichment of genes involved in the inflammatory response, cell signalling pathways and apoptosis. The transcriptional changes associated with cellular signalling and the inflammatory response may reflect different immuno-modulatory mechanisms that underlie host-pathogen interactions during infection.
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