ArticleEvidence-based complementary and alternative medicine : eCAM2012
The Antiproliferative Activity of Sclerotia of Lignosus rhinocerus (Tiger Milk Mushroom).
Article in Evidence-based complementary and alternative medicine : eCAM, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Efficacy and safety of Lignosus rhinocerus (Cooke) Ryvarden TM02BMC complementary medicine and therapies · 2025Trial
- Extract optimization and biological activities of Otidea onotica using Artificial Neural Network-Genetic Algorithm and response surface methodology techniques.BMC biotechnology · 2025Article
- Current Advances in the Use of Mushrooms as Therapeutics for Lung Cancer: A Review.Molecules (Basel, Switzerland) · 2025Review
- Review
- Comparative Cytotoxicity Evaluation of Heat-AssistedFood technology and biotechnology · 2024Article
- Immunomodulatory Effect and an Intervention of TNF Signalling Leading to Apoptotic and Cell Cycle Arrest on ORL-204 Oral Cancer Cells by Tiger Milk Mushroom,Food technology and biotechnology · 2022Article
- Effect of tiger milk mushroom (Lignosus rhinocerus) supplementation on respiratory health, immunity and antioxidant status: an open-label prospective study.Scientific reports · 2021Article
- Nutritional and 13-Week Subchronic Toxicological Evaluation ofInternational journal of environmental research and public health · 2021Article
- Proteins fromPeerJ · 2020Article
- Antibacterial activity of biosynthesized gold nanoparticles using biomolecules fromSaudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society · 2019Article
- Human Embryonic Stem Cell-Derived Neural Lineages asBioMed research international · 2019Article
- Airway Relaxation Effects of Water-Soluble Sclerotial Extract FromFrontiers in pharmacology · 2018Article
- Inhibition of Protein Glycation by Tiger Milk Mushroom [Frontiers in pharmacology · 2018Article
- Article
- Comparative nutritional and mycochemical contents, biological activities and LC/MS screening of tuber from new recipe cultivation technique with wild type tuber of tiger's milk mushroom of species Lignosus rhinocerus.World journal of microbiology & biotechnology · 2017Article
- Heterologous expression of cytotoxic sesquiterpenoids from the medicinal mushroom Lignosus rhinocerotis in yeast.Microbial cell factories · 2017Article
- A Status Review of the Bioactive Activities of Tiger Milk MushroomFrontiers in pharmacology · 2017Review
- Safety Evaluation of Sclerotium from a Medicinal Mushroom,Frontiers in pharmacology · 2017Article
- Characterisation of a New Fungal Immunomodulatory Protein from Tiger Milk mushroom, Lignosus rhinocerotis.Scientific reports · 2016Article
- Sub-Acute Toxicity Study of Tiger Milk Mushroom Lignosus tigris Chon S. Tan Cultivar E Sclerotium in Sprague Dawley Rats.Frontiers in pharmacology · 2016Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lignosus rhinocerus, the tiger milk mushroom, is one of the most important medicinal mushrooms used by the indigenous people of Southeast Asia and China. It has been used to treat breast cancer. A cold water extract (LR-CW) prepared from the sclerotia of L. rhinocerus cultivar was found to exhibit antiproliferative activity against human breast carcinoma (MCF-7) and human lung carcinoma (A549), with IC(50) of 96.7 μg/mL and 466.7 μg/mL, respectively. In comparison, LR-CW did not show significant cytotoxicity against the two corresponding human normal cells, 184B5 (human breast cell) and NL 20 (human lung cell). DNA fragmentation studies suggested that the cytotoxic action of LR-CW against cancer cells is mediated by apoptosis. Sephadex G-50 gel filtration fractionation of LR-CW yielded a high-molecular-weight and a low-molecular-weight fraction. The high-molecular-weight fraction contains mainly carbohydrate (68.7%) and small amount of protein (3.6%), whereas the low-molecular-weight fraction contains 31% carbohydrate and was devoid of protein. Only the high-molecular-weight fraction exhibited antiproliferative activity against cancer cells, with IC(50) of 70.0 μg/mL and 76.7 μg/mL, respectively. Thus, the cytotoxic action of the LR-CW is due to the high-molecular-weight fraction, either the proteins or protein-carbohydrate complex.
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Registered trials
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