ArticleJournal of visualized experiments : JoVE2012
Transretinal ERG recordings from mouse retina: rod and cone photoresponses.
Article in Journal of visualized experiments : JoVE, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 22 citations in OpenAlex.
- Chromophore-loaded CRALBP mutant proteins restore rod function in chromophore-deficient mice.Molecular therapy. Advances · 2026Article
- Insights into rhodopsin molecular evolution from mice with "humanized" Phe-88 to Leu substitution.Research square · 2025Article
- Photoreceptor deficits appear at eye opening in Rs1 mutant mouse models of X-linked retinoschisis.Experimental eye research · 2024Article
- Light drives the developmental progression of outer retinal function.The Journal of general physiology · 2023Article
- An Ex Vivo Electroretinographic Apparatus for the mL-Scale Testing of Drugs to One Day and Beyond.International journal of molecular sciences · 2023Article
- Redox-Responsive Hyaluronic Acid-Based Nanogels for the Topical Delivery of the Visual Chromophore to Retinal Photoreceptors.ACS omega · 2021Article
- Ex vivo electroretinograms made easy: performing ERGs using 3D printed components.The Journal of physiology · 2020Article
- Mislocalization of cone nuclei impairs cone function in mice.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Photoreceptors in a mouse model of Leigh syndrome are capable of normal light-evoked signaling.The Journal of biological chemistry · 2019Article
- Myopia disease mouse models: a missense point mutation (S673G) and a protein-truncating mutation of theCell & bioscience · 2019Article
- Examining the Role of Cone-expressed RPE65 in Mouse Cone Function.Scientific reports · 2018Article
- Where You Cut Matters: A Dissection and Analysis Guide for the Spatial Orientation of the Mouse Retina from Ocular Landmarks.Journal of visualized experiments : JoVE · 2018Article
- A novel map of the mouse eye for orienting retinal topography in anatomical space.The Journal of comparative neurology · 2018Article
- The Development of Mid-Wavelength Photoresponsivity in the Mouse Retina.Current eye research · 2018Article
- The Contribution of L-Type CaFrontiers in molecular neuroscience · 2017Article
- Differential stimulation of the retina with subretinally injected exogenous neurotransmitter: A biomimetic alternative to electrical stimulation.Scientific reports · 2016Article
- The B3 Subunit of the Cone Cyclic Nucleotide-gated Channel Regulates the Light Responses of Cones and Contributes to the Channel Structural Flexibility.The Journal of biological chemistry · 2016Article
- Systemic Retinaldehyde Treatment Corrects Retinal Oxidative Stress, Rod Dysfunction, and Impaired Visual Performance in Diabetic Mice.Investigative ophthalmology & visual science · 2015Article
- Simultaneous ex vivo functional testing of two retinas by in vivo electroretinogram system.Journal of visualized experiments : JoVE · 2015Article
- Retinal cone photoreceptors require phosducin-like protein 1 for G protein complex assembly and signaling.PloS one · 2015Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
There are two distinct classes of image-forming photoreceptors in the vertebrate retina: rods and cones. Rods are able to detect single photons of light whereas cones operate continuously under rapidly changing bright light conditions. Absorption of light by rod- and cone-specific visual pigments in the outer segments of photoreceptors triggers a phototransduction cascade that eventually leads to closure of cyclic nucleotide-gated channels on the plasma membrane and cell hyperpolarization. This light-induced change in membrane current and potential can be registered as a photoresponse, by either classical suction electrode recording technique or by transretinal electroretinogram recordings (ERG) from isolated retinas with pharmacologically blocked postsynaptic response components. The latter method allows drug-accessible long-lasting recordings from mouse photoreceptors and is particularly useful for obtaining stable photoresponses from the scarce and fragile mouse cones. In the case of cones, such experiments can be performed both in dark-adapted conditions and following intense illumination that bleaches essentially all visual pigment, to monitor the process of cone photosensitivity recovery during dark adaptation. In this video, we will show how to perform rod- and M/L-cone-driven transretinal recordings from dark-adapted mouse retina. Rod recordings will be carried out using retina of wild type (C57Bl/6) mice. For simplicity, cone recordings will be obtained from genetically modified rod transducin α-subunit knockout (Tα(-/-)) mice which lack rod signaling(8).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.