Evidence map›Paper›PMID 22453101›Full record

ArticleHead & neck oncology2012

Nimesulide inhibited the growth of hypopharyngeal carcinoma cells via suppressing Survivin expression.

Tian Jia-Jun, Lu Su-Mei, Yu Liang, Ma Ju-Ke, Mu Ya-Kui, Wang Hai-Bo, Xu Wei

Expression of concernOpen access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Head & neck oncology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It carries an expression of concern. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.5field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 16 citations in OpenAlex.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Tian Jia-Jun *Department of Otolaryngology-Head and Neck Surgery, Provincial Hospital affiliated to Shandong University, Jinan 250021, PR, China.
Lu Su-Mei *Institute of Eye & Otolaryngology, Shandong Clinic Research Institute, Jinan 250021, PR, China.
Yu LiangDepartment of Otolaryngology-Head and Neck Surgery, Provincial Hospital affiliated to Shandong University, Jinan 250021, PR, China.
Ma Ju-KeDepartment of Otolaryngology-Head and Neck Surgery, Provincial Hospital affiliated to Shandong University, Jinan 250021, PR, China.
Mu Ya-KuiDepartment of Otolaryngology-Head and Neck Surgery, Provincial Hospital affiliated to Shandong University, Jinan 250021, PR, China.
Wang Hai-BoDepartment of Otolaryngology-Head and Neck Surgery, Provincial Hospital affiliated to Shandong University, Jinan 250021, PR, China.
Xu WeiDepartment of Otolaryngology-Head and Neck Surgery, Provincial Hospital affiliated to Shandong University, Jinan 250021, PR, China.
Shandong University · CNShandong Eye Hospital · CNShandong Provincial Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe objective of this study was to evaluate the efficacy of Nimesulide, a selective cyclooxygenase-2 (COX-2) inhibitor, on the growth of hypopharyngeal carcinoma cells (FaDu) in vitro, and investigate its potential mechanism.

methodsAfter FaDu cells were treated with graded concentrations of Nimesulide for divergent time, sensitivity of cells to drug treatment was analyzed by MTT assay. Morphological changes of FaDu cells in the presence of Nimesulide were observed by acridine orange cytochemistry staining. Proliferating cells were detected using the 5-Bromo-2'-deoxy-uridine (BrdU) incorporation assay. Following cells were subjected to Nimesulide (500 μmol/l) for 6 h, 12 h and 24 h, the percentage of apoptosis was examined by flow cytometry. We detected COX-2 and Survivin expression change by RT-PCR and Western blot, and analyzed the correlation of them with the growth of FaDu cells. Additionally, we also analyzed Caspase-3, Bcl-2 and Bax expressions as markers to investigate the related pathway of Nimesulide-indued apoptosis.

resultsCompared with the control group, the viabilities rates were decreased by Nimesulide in time- and dose-dependent manners, typical morphological changes of apoptotic cells were observed in the Nimesulide-treatment groups, Nimesulide could suppress the proliferation of FaDu cells significantly. The percentage of apoptosis in FaDu cells were markedly increased after Nimesulide-treatment for 6 h, 12 h and 24 h. Nimesulide down-regulated the Survivin and COX-2 expressions at mRNA and protein levels in FaDu cells. Additional analyses indicated that Bcl-2 expression was significantly decreased and the expressions of Caspase-3 as well as Bax were increased at both mRNA and protein levels.

conclusionsBased on the induction of apoptosis and suppression of proliferation, Nimesulide could inhibit the growth of FaDu cells. Furthermore, the suppression of Survivin expression may play an important role in Nimesulide-induced growth inhibition. Nimesulide could act as an effective therapeutic agent for hypopharyngeal carcinoma therapy.

Indexed as

Carcinoma, Squamous CellCell Growth ProcessesCell Line, TumorCyclooxygenase 2 InhibitorsDose-Response Relationship, DrugHead and Neck NeoplasmsHumansHypopharyngeal NeoplasmsInhibitor of Apoptosis ProteinsSquamous Cell Carcinoma of Head and NeckSulfonamidesSurvivinBIRC5 protein, humanCyclooxygenase 2 InhibitorsInhibitor of Apoptosis ProteinsnimesulideSulfonamidesSurvivin

Identifiers

PMID22453101
PMCPMC3364892
OpenAlexW2157380728

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.