ArticleJournal of experimental & clinical cancer research : CR2012
Up-regulation of hypoxia inducible factor-1α by cobalt chloride correlates with proliferation and apoptosis in PC-2 cells.
Article in Journal of experimental & clinical cancer research : CR, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 94 citations in OpenAlex.
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- Trace elements in pancreatic cancer.Cancer medicine · 2024Review
- Potential Impact of Hypoxic Astrocytes on the Aggravation of Depressive Symptoms in Parkinson's Disease.Journal of molecular neuroscience : MN · 2024Article
- Trace element concentrations in blood samples from dairy cows with uterine torsion and their neonatal calves.Veterinary world · 2023Article
- Host cell egress of Brucella abortus requires BNIP3L-mediated mitophagy.The EMBO journal · 2023Article
- Hypoxia represses FOXF1 in lung endothelial cells through HIF-1α.Frontiers in physiology · 2023Article
- Acute vs. chronic vs. intermittent hypoxia in breast Cancer: a review on its application in in vitro research.Molecular biology reports · 2022Review
- Ultraviolet Radiation Promoted Hypoxia-Induced Apoptosis in HL-60 Human Promyelocytic Leukemia Cell Line.Journal of oncology · 2022Article
- FAM13A promotes proliferation of bovine preadipocytes by targeting Hypoxia-Inducible factor-1 signaling pathway.Adipocyte · 2021Article
- Genomic organization and hypoxia inducible factor responsive regulation of teleost hsp90β gene during hypoxia stress.Molecular biology reports · 2021Article
- Squalene deters drivers of RCC disease progression beyond VHL status.Cell biology and toxicology · 2021Article
- Engineering Tools for Regulating Hypoxia in Tumour Models.Journal of cellular and molecular medicine · 2021Review
- A Straightforward Hypoxic Cell Culture Method Suitable for Standard Incubators.Methods and protocols · 2021Article
- Development of a cobalt(iii)-based ponatinib prodrug system.Inorganic chemistry frontiers · 2021Article
- Development and validation of a quantitative electron microscopy score to assess acute cellular stress in the human exocrine pancreas.The journal of pathology. Clinical research · 2021Article
- Airway stem cells sense hypoxia and differentiate into protective solitary neuroendocrine cells.Science (New York, N.Y.) · 2021Article
- Hypoxia Induced by Cobalt Chloride Triggers Autophagic Apoptosis of Human and Mouse Drug-Resistant Glioblastoma Cells through Targeting the PI3K-AKT-mTOR Signaling Pathway.Oxidative medicine and cellular longevity · 2021Article
- Improving the Stability of EGFR Inhibitor Cobalt(III) Prodrugs.Inorganic chemistry · 2020Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe exact mechanism of the effects of hypoxia on the proliferation and apoptosis in carcinoma cells is still conflicting. This study investigated the variation of hypoxia-inducible factor-1α(HIF-1α) expression and the apoptosis effect of hypoxia stimulated by cobalt chloride (CoCl(2)) in pancreatic cancer PC-2 cells.
methodsPC-2 cells were cultured with different concentration (50-200 μmol/L) of CoCl(2) after 24-120 hours to simulate hypoxia in vitro. The proliferation of PC-2 cells was examined by MTT assay. The cellular morphology of PC-2 cells were observed by light inverted microscope and transmission electron microscope(EM). The expression of HIF-1α on mRNA and protein level was measured by semi-quantitative RT-PCR and Western blot analysis. Apoptosis of PC-2 cells were demonstrated by flow cytometry with Annexin V-FITC/PI double staining.
resultsMTT assay showed that the proliferation of PC-2 cells were stimulated in the first 72 h, while after treated over 72 h, a dose- dependent inhibition of cell growth could be observed. By using transmission electron microscope, swollen chondrosomes, accumulated chromatin under the nuclear membrane and apoptosis bodies were observed. Flow cytometer(FCM) analysis showed the apoptosis rate was correlated with the dosage of CoCl(2). RT-PCR and Western blot analysis indicated that hypoxia could up-regulate the expression of HIF-1α on both mRNA and protein levels.
conclusionHypoxic microenvironment stimulated by CoCl(2) could effectively induce apoptosis and influence cell proliferation in PC-2 cells, the mechanism could be related to up-expression of HIF-1α.
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