ArticleEnvironmental health : a global access science source2012
Activation of inflammatory responses in human U937 macrophages by particulate matter collected from dairy farms: an in vitro expression analysis of pro-inflammatory markers.
Article in Environmental health : a global access science source, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- Trial
- Secretome From Human Micro-Fragmented Adipose Tissue Affects In Vitro Monocytes/Macrophages Inflammatory Activity by ICAM-1 Expression.Mediators of inflammation · 2026Article
- Cell-based evaluation of anti-inflammatory activity from the combination of natural compounds in LPS-stimulated U937 monocytes.Cytotechnology · 2025Article
- The Anti-Inflammatory Effects ofMolecules (Basel, Switzerland) · 2023Article
- The retina-specific basigin isoform does not induce IL-6 expression in mouse monocytes.Molecular vision · 2023Article
- A comparative study on the model of PMEnvironmental science and pollution research international · 2022Article
- Chemical and Toxicological Properties of Emissions from a Light-Duty Compressed Natural Gas Vehicle Fueled with Renewable Natural Gas.Environmental science & technology · 2021Article
- In vivo and in vitro inflammatory responses to fine particulate matter (PMToxicology letters · 2020Article
- Ex vivo innate responses to particulate matter from livestock farms in asthma patients and healthy individuals.Environmental health : a global access science source · 2020Article
- Composition and Toxicity of Biogas Produced from Different Feedstocks in California.Environmental science & technology · 2019Article
- Organic dust induces inflammatory gene expression in lung epithelial cells via ROS-dependent STAT-3 activation.American journal of physiology. Lung cellular and molecular physiology · 2019Article
- PMInternational journal of medical sciences · 2019Article
- Black Walnut (Frontiers in pharmacology · 2019Article
- Personal exposure of dairy workers to dust, endotoxin, muramic acid, ergosterol, and ammonia on large-scale dairies in the high plains Western United States.Journal of occupational and environmental hygiene · 2018Article
- Chemokine CXCL8 promotes HIV-1 replication in human monocyte-derived macrophages and primary microglia via nuclear factor-κB pathway.PloS one · 2014Article
- Regulatory T cells protect fine particulate matter-induced inflammatory responses in human umbilical vein endothelial cells.Mediators of inflammation · 2014Article
- Thrombospondin 1 activates the macrophage Toll-like receptor 4 pathway.Cellular & molecular immunology · 2013Article
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe purpose of the present study was to investigate activation of inflammatory markers in human macrophages derived from the U937 cell line after exposure to particulate matter (PM) collected on dairy farms in California and to identify the most potent components of the PM.
methodsPM from different dairies were collected and tested to induce an inflammatory response determined by the expression of various pro-inflammatory genes, such as Interleukin (IL)-8, in U937 derived macrophages. Gel shift and luciferase reporter assays were performed to examine the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and Toll-like-receptor 4 (TLR4).
resultsMacrophage exposure to PM derived from dairy farms significantly activated expression of pro-inflammatory genes, including IL-8, cyclooxygenase 2 and Tumor necrosis factor-alpha, which are hallmarks of inflammation. Acute phase proteins, such as serum amyloid A and IL-6, were also significantly upregulated in macrophages treated with PM from dairies. Coarse PM fractions demonstrated more pro-inflammatory activity on an equal-dose basis than fine PM. Urban PM collected from the same region as the dairy farms was associated with a lower concentration of endotoxin and produced significantly less IL-8 expression compared to PM collected on the dairy farms.
conclusionThe present study provides evidence that the endotoxin components of the particles collected on dairies play a major role in mediating an inflammatory response through activation of TLR4 and NF-κB signaling.
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