ArticleProceedings of the National Academy of Sciences of the United States of America2012
An essential bacterial-type cardiolipin synthase mediates cardiolipin formation in a eukaryote.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 57 citations in OpenAlex.
- A prokaryotic-like cardiolipin synthase is essential to maintain mitochondrial function, lipid homeostasis, and survival of the blood stage malaria parasite Plasmodium falciparum.The Journal of biological chemistry · 2026Article
- A bacterial-type cardiolipin synthase in Plasmodium spp. supports mitochondrial respiration and is important for liver stage maturation.PLoS pathogens · 2026Article
- Unconventional model organisms bend our view on mitochondrial cristae.Journal of cell science · 2026Review
- Origin and evolution of mitochondrial inner membrane composition.Journal of cell science · 2025Review
- Evaluation of theACS infectious diseases · 2024Article
- Multiple approaches of cellular metabolism define the bacterial ancestry of mitochondria.Science advances · 2023Article
- Identification of TbPBN1 in Trypanosoma brucei reveals a conserved heterodimeric architecture for glycosylphosphatidylinositol-mannosyltransferase-I.Molecular microbiology · 2022Article
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- Eugene P. Kennedy's Legacy: Defining Bacterial Phospholipid Pathways and Function.Frontiers in molecular biosciences · 2021Review
- A Conserved Mitochondrial Chaperone-Protease Complex Involved in Protein Homeostasis.Frontiers in molecular biosciences · 2021Article
- Metabolic Alterations Caused by Defective Cardiolipin Remodeling in Inherited Cardiomyopathies.Life (Basel, Switzerland) · 2020Review
- The mitochondrial peroxiredoxin displays distinct roles in different developmental stages of African trypanosomes.Redox biology · 2020Article
- Mitochondrial sphingosine-1-phosphate lyase is essential for phosphatidylethanolamine synthesis and survival of Trypanosoma brucei.Scientific reports · 2020Article
- Metabolic Alterations in Inherited Cardiomyopathies.Journal of clinical medicine · 2019Review
- Lipid analysis ofCell discovery · 2018Article
- Cross-species complementation of bacterial- and eukaryotic-type cardiolipin synthases.Microbial cell (Graz, Austria) · 2017Article
- Two phylogenetically and compartmentally distinct CDP-diacylglycerol synthases cooperate for lipid biogenesis inThe Journal of biological chemistry · 2017Article
- Biosynthesis, remodeling and turnover of mitochondrial cardiolipin.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2017Review
- Lipid topogenesis--35years on.Biochimica et biophysica acta · 2016Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cardiolipin is important for bacterial and mitochondrial stability and function. The final step in cardiolipin biosynthesis is catalyzed by cardiolipin synthase and differs mechanistically between prokaryotes and eukaryotes. To study the importance of cardiolipin synthesis for mitochondrial integrity, membrane protein complex formation, and cell proliferation in the human and animal pathogenic protozoan parasite, Trypanosoma brucei, we generated conditional cardiolipin synthase-knockout parasites. We found that cardiolipin formation in T. brucei procyclic forms is catalyzed by a bacterial-type cardiolipin synthase, providing experimental evidence for a prokaryotic-type cardiolipin synthase in a eukaryotic organism. Ablation of enzyme expression resulted in inhibition of de novo cardiolipin synthesis, reduction in cellular cardiolipin levels, alterations in mitochondrial morphology and function, and parasite death in culture. By using immunofluorescence microscopy and blue-native gel electrophoresis, cardiolipin synthase was shown to colocalize with inner mitochondrial membrane proteins and to be part of a large protein complex. During depletion of cardiolipin synthase, the levels of cytochrome oxidase subunit IV and cytochrome c1, reflecting mitochondrial respiratory complexes IV and III, respectively, decreased progressively.
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