ArticleProceedings of the National Academy of Sciences of the United States of America2012
Single-molecule observation of helix staggering, sliding, and coiled coil misfolding.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed.
- Nontoxic mRNA vehicles based on electrically neutral self-assembling peptides.Nature communications · 2026Article
- Co-translational ribosome pairing enables native assembly of misfolding-prone subunits.Nature communications · 2025Article
- Construction of Nanotube-Shaped mRNA Vehicles Using Self-Assembling Peptides.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Force redistribution in clathrin-mediated endocytosis revealed by coiled-coil force sensors.Science advances · 2023Article
- Single-molecule experiments reveal the elbow as an essential folding guide in SMC coiled-coil arms.Biophysical journal · 2022Article
- Energetics, kinetics, and pathways of SNARE assembly in membrane fusion.Critical reviews in biochemistry and molecular biology · 2022Review
- Chirality transmission in macromolecular domains.Nature communications · 2022Article
- Coiled-coil registry shifts in the F684I mutant of Bicaudal D result in cargo-independent activation of dynein motility.Traffic (Copenhagen, Denmark) · 2020Article
- Chaperone-Assisted Host-Guest Interactions Revealed by Single-Molecule Force Spectroscopy.Journal of the American Chemical Society · 2019Article
- Role of Coiled-Coil Registry Shifts in the Activation of Human Bicaudal D2 for Dynein Recruitment upon Cargo Binding.The journal of physical chemistry letters · 2019Article
- Sliding Mechanism at a Coiled-Coil Interface.Biophysical journal · 2019Article
- Article
- Molecular mechanics of coiled coils loaded in the shear geometry.Chemical science · 2018Article
- Energetics, kinetics, and pathway of SNARE folding and assembly revealed by optical tweezers.Protein science : a publication of the Protein Society · 2017Review
- Single-Molecule Protein Folding Experiments Using High-Precision Optical Tweezers.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Pharmacological chaperone reshapes the energy landscape for folding and aggregation of the prion protein.Nature communications · 2016Article
- Structure-Based Derivation of Protein Folding Intermediates and Energies from Optical Tweezers.Biophysical journal · 2016Article
- Article
- Probing the structural dynamics of proteins and nucleic acids with optical tweezers.Current opinion in structural biology · 2015Review
- Kinetically coupled folding of a single HIV-1 glycoprotein 41 complex in viral membrane fusion and inhibition.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The biological functions of coiled coils generally depend on efficient folding and perfect pairing of their α-helices. Dynamic changes in the helical registry that lead to staggered helices have only been proposed for a few special systems and not found in generic coiled coils. Here, we report our observations of multiple staggered helical structures of two canonical coiled coils. The partially folded structures are formed predominantly by coiled coil misfolding and occasionally by helix sliding. Using high-resolution optical tweezers, we characterized their energies and transition kinetics at a single-molecule level. The staggered states occur less than 2% of the time and about 0.1% of the time at zero force. We conclude that dynamic changes in helical registry may be a general property of coiled coils. Our findings should have broad and unique implications in functions and dysfunctions of proteins containing coiled coils.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.