ArticleMolecular and cellular biology2012
Rapid cytoplasmic turnover of yeast ribosomes in response to rapamycin inhibition of TOR.
Article in Molecular and cellular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 48 citations in OpenAlex.
- RiboScreenBiomedicines · 2026Review
- Measurement of rRNA Synthesis and Degradation Rates byMethods in molecular biology (Clifton, N.J.) · 2025Article
- Plant ribosomes as a score to fathom the melody of 2'-RNA biology · 2024Review
- Patterns of protein synthesis in the budding yeast cell cycle: variable or constant?Microbial cell (Graz, Austria) · 2024Article
- Differential Hsp90-dependent gene expression is strain-specific and common among yeast strains.iScience · 2023Article
- Deubiquitinase Ubp3 regulates ribophagy and deubiquitinates Smo1 for appressorium-mediated infection by Magnaporthe oryzae.Molecular plant pathology · 2022Article
- Dot6/Tod6 degradation fine-tunes the repression of ribosome biogenesis under nutrient-limited conditions.iScience · 2022Article
- Modeling in yeast how rDNA introns slow growth and increase desiccation tolerance in lichens.G3 (Bethesda, Md.) · 2021Article
- Unexpected Role of Sterol Synthesis in RNA Stability and Translation inBiomedicines · 2021Article
- Proteomics Reveals Distinct Changes Associated with Increased Gamma Radiation Resistance in the Black YeastGenes · 2020Article
- Cell- and Polymerase-Selective Metabolic Labeling of Cellular RNA with 2'-Azidocytidine.Journal of the American Chemical Society · 2020Article
- Metabolic labeling of RNA uncovers the contribution of transcription and decay rates on hypoxia-induced changes in RNA levels.RNA (New York, N.Y.) · 2020Article
- Proteotoxic stress promotes entrapment of ribosomes and misfolded proteins in a shared cytosolic compartment.Nucleic acids research · 2020Article
- Nuclear Argonaute Piwi Gene Mutation Affects rRNA by Inducing rRNA Fragment Accumulation, Antisense Expression, and Defective Processing inInternational journal of molecular sciences · 2020Article
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- Mechanisms by which PE21, an extract from the white willowOncotarget · 2019Article
- Protein synthesis and quality control in aging.Aging · 2018Review
- Feedback regulation of ribosome assembly.Current genetics · 2018Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 2 countries.
Funding
Abstract
The target of rapamycin (TOR) pathway is the central regulator of cell growth in eukaryotes. Inhibition of TOR by rapamycin elicits changes in translation attributed mainly to altered translation initiation and repression of the synthesis of new ribosomes. Using quantitative analysis of rRNA, we found that the number of existing ribosomes present in a Saccharomyces cerevisiae culture during growth in rich medium rapidly decreases by 40 to 60% when the cells are treated with rapamycin. This process is not appreciably affected by a suppression of autophagy, previously implicated in degradation of ribosomes in eukaryotes upon starvation. Yeast cells deficient in the exosome function or lacking its cytoplasmic Ski cofactors show an abnormal pattern of rRNA degradation, particularly in the large ribosomal subunit, and accumulate rRNA fragments after rapamycin treatment and during diauxic shift. The exosome and Ski proteins are thus important for processing of rRNA decay intermediates, although they are probably not responsible for initiating rRNA decay. The role of cytoplasmic nucleases in rapamycin-induced rRNA degradation suggests mechanistic parallels of this process to nutrient-controlled ribosome turnover in prokaryotes. We propose that ribosome content is regulated dynamically in eukaryotes by TOR through both ribosome synthesis and the cytoplasmic turnover of mature ribosomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.