ArticleThe Journal of physiology2012
Bleaching of mouse rods: microspectrophotometry and suction-electrode recording.
Article in The Journal of physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 48 citations in OpenAlex.
- E122Q rhodopsin: pigment microspectrophotometry, photoreceptor light responses, and bleaching adaptation.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2026Article
- Functional OCT with parfocal retinal stimulation enables multi-site optoretinography under a single dark adaptation.Biomedical optics express · 2026Article
- Rhodopsin molecular evolution from mouse to human phenylalanine 88 to leucine substitution enhances thermal stability and post-activation decay.Scientific reports · 2026Article
- The physiology of dark adaptation: Progress and future directions.Progress in retinal and eye research · 2025Review
- Insights into rhodopsin molecular evolution from mice with "humanized" Phe-88 to Leu substitution.Research square · 2025Article
- The Role of the CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Tunable anti-ambipolar vertical bilayer organic electrochemical transistor enable neuromorphic retinal pathway.Nature communications · 2024Article
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- Light Adaptation of Retinal Rod Bipolar Cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- A Novel Role for UNC119 as an Enhancer of Synaptic Transmission.International journal of molecular sciences · 2023Article
- Adaptation memory in photoreceptors: different mechanisms in rods and cones.Frontiers in molecular neuroscience · 2023Article
- Reproducibility of the Rod Photoreceptor Response Depends Critically on the Concentration of the Phosphodiesterase Effector Enzyme.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- Light responses of mammalian cones.Pflugers Archiv : European journal of physiology · 2021Review
- Rod Photoreceptors Avoid Saturation in Bright Light by the Movement of the G Protein Transducin.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Apo-Opsin and Its Dark Constitutive Activity across Retinal Cone Subtypes.Current biology : CB · 2020Article
- Rhodopsin-mediated light-off-induced protein kinase A activation in mouse rod photoreceptor cells.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Photic generation of 11-The Journal of biological chemistry · 2019Article
- Light-Driven Regeneration of Cone Visual Pigments through a Mechanism Involving RGR Opsin in Müller Glial Cells.Neuron · 2019Article
- Elementary response triggered by transducin in retinal rods.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
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5 authors at 3 institutions in 1 country.
Funding
Abstract
When a substantial fraction of rhodopsin in a rod photoreceptor is exposed to bright light, the rod is desensitized by a process known as bleaching adaptation. Experiments on isolated photoreceptors in amphibians have revealed many of the features of bleaching adaptation, but such experiments have not so far been possible in mammals. We now describe a method for making microspectrophotometric measurements of pigment concentration and suction-electrode recording of electrical responses over a wide range of bleaching exposures from isolated mouse rods or pieces of mouse retina. We show that if pigment is bleached at a low rate in the presence of bovine serum albumin (BSA), and intermediate photoproducts are allowed to decay, mouse rods are stably desensitized; subsequent treatment with exogenous 11-cis retinal results in pigment regeneration and substantial recovery of sensitivity to the dark-adapted value. Stably bleached wild-type (WT) rods show a decrease in circulating current and acceleration of the time course of decay, much as in steady background light; similar effects are seen in guanylyl cyclase-activating protein knockout (GCAPs(-/-)) rods, indicating that regulation of guanylyl cyclase is not necessary for at least a part of the adaptation produced by bleaching. Our experiments demonstrate that in mammalian rods, as in amphibian rods, steady-state desensitization after bleaching is produced by two components: (1) a reduction in the probability of photon absorption produced by a decrease in rhodopsin concentration; and (2) an equivalent background light whose intensity is proportional to the fraction of bleached pigment, and which adapts the rod like real background light. These two mechanisms together fully account for the ‘log-linear' relationship in mammalian retina between sensitivity and per cent bleach, which can be measured in the steady state following exposure to bright light. Our methods will now make possible an examination of bleaching adaptation and pigment regeneration in mouse animal lines with mutations or other alterations in the proteins of transduction.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.