Evidence map›Paper›PMID 22450983›Full record

ArticleAntimicrobial agents and chemotherapy2012

Multiplex real-time PCR for detection of an epidemic KPC-producing Klebsiella pneumoniae ST258 clone.

Liang Chen, Kalyan D Chavda, José R Mediavilla, Yanan Zhao, Henry S Fraimow, Stephen G Jenkins, Michael H Levi, Tao Hong, Albert D Rojtman, Christine C Ginocchio and 2 more

Open access · bronzeAbstract read
In one paragraph

Article in Antimicrobial agents and chemotherapy, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.

0numbers the graph read from it
0cells of the map it votes in
37citing papers in PubMed
3.0field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

37 citing papers in PubMed, 62 citations in OpenAlex.

  1. Molecular Insights into Carbapenem Resistance inInternational journal of molecular sciences · 2026
    Review
  2. Detection ofMicroorganisms · 2025
    Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Effects of KPC Variant and Porin Genotype on theAntimicrobial agents and chemotherapy · 2019
    Article
  12. Observational
  13. Article
  14. Review
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 9 institutions in 1 country.

Liang ChenPublic Health Research Institute Center, International Center for Public Health, University of Medicine and Dentistry of New Jersey, Newark, New Jersey, USA.
Kalyan D Chavda
José R Mediavilla
Yanan Zhao
Henry S Fraimow
Stephen G Jenkins
Michael H Levi
Tao Hong
Albert D Rojtman
Christine C Ginocchio
Robert A Bonomo
Barry N Kreiswirth
Rutgers, The State University of New Jersey · USInternational Center for Public Health · USCooper Medical School of Rowan University · USCornell University · USHackensack University Medical Center · USJersey Shore University Medical Center · USLouis Stokes Cleveland VA Medical Center · USMontefiore Medical Center · USNorthwell Health · US

Funding

The molecular basis of the epidemic blaKPC gene KlebsiellaR01AI090155 · NIAID · UNIV OF MED/DENT OF NJ-NJ MEDICAL SCHOOL · PI KREISWIRTH, BARRY NEAL · 2011 to 2021
$7.2M
Understanding ceftazidime resistance in SHV B-LactamasesR01AI063517 · NIAID · CASE WESTERN RESERVE UNIVERSITY · PI BONOMO, ROBERT A. · 2005 to 2023
$4.9M
NIAID NIH HHS 1R01AI090155-01A1NIAID NIH HHS R01 AI090155
6 · The paper itself

Abstract

We describe a multiplex real-time PCR assay capable of identifying both the epidemic Klebsiella pneumoniae ST258 clone and bla(KPC) carbapenemase genes in a single reaction. The assay displayed excellent sensitivity (100%) and specificity (100%) for identification of ST258 clone and bla(KPC) in a collection of 75 K. pneumoniae isolates comprising 41 sequence types. Our results suggest that this assay is an effective tool for surveillance of this clone among carbapenem-resistant K. pneumoniae clinical isolates.

Indexed as

Anti-Bacterial AgentsBacterial Proteinsbeta-LactamasesCarbapenemsDrug Resistance, Multiple, BacterialKlebsiella pneumoniaeReal-Time Polymerase Chain ReactionAnti-Bacterial AgentsBacterial Proteinsbeta-LactamasescarbapenemaseCarbapenems

Identifiers

PMID22450983
PMCPMC3370784
OpenAlexW2025137526

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.