ArticleRNA (New York, N.Y.)2012
Thermodynamic characterization of the Saccharomyces cerevisiae telomerase RNA pseudoknot domain in vitro.
Article in RNA (New York, N.Y.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Article
- Article
- Inverse-Folding Design of Yeast Telomerase RNA Increases Activity In Vitro.Non-coding RNA · 2023Article
- Inverse-folding design of yeast telomerase RNA increases activitybioRxiv : the preprint server for biology · 2023Article
- Yeast Telomerase RNA Flexibly Scaffolds Protein Subunits: Results and Repercussions.Molecules (Basel, Switzerland) · 2020Review
- Mechanisms of template handling and pseudoknot folding in human telomerase and their manipulation to expand the sequence repertoire of processive repeat synthesis.Nucleic acids research · 2018Article
- Structure and folding of the Tetrahymena telomerase RNA pseudoknot.Nucleic acids research · 2017Article
- Refined secondary-structure models of the core of yeast and human telomerase RNAs directed by SHAPE.RNA (New York, N.Y.) · 2015Article
- RNA connectivity requirements between conserved elements in the core of the yeast telomerase RNP.The EMBO journal · 2013Article
- Pyrimidine motif triple helix in the Kluyveromyces lactis telomerase RNA pseudoknot is essential for function in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Identification of telomerase RNAs from filamentous fungi reveals conservation with vertebrates and yeasts.PloS one · 2013Article
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Authors and funding
4 authors.
Funding
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Abstract
Recent structural and functional characterization of the pseudoknot in the Saccharomyces cerevisiae telomerase RNA (TLC1) has demonstrated that tertiary structure is present, similar to that previously described for the human and Kluyveromyces lactis telomerase RNAs. In order to biophysically characterize the identified pseudoknot secondary and tertiary structures, UV-monitored thermal denaturation experiments, nuclear magnetic resonance spectroscopy, and native gel electrophoresis were used to investigate various potential conformations in the pseudoknot domain in vitro, in the absence of the telomerase protein. Here, we demonstrate that alternative secondary structures are not mutually exclusive in the S. cerevisiae telomerase RNA, tertiary structure contributes 1.5 kcal mol(-1) to the stability of the pseudoknot (≈ half the stability observed for the human telomerase pseudoknot), and identify additional base pairs in the 3' pseudoknot stem near the helical junction. In addition, sequence conservation in an adjacent overlapping hairpin appears to prevent dimerization and alternative conformations in the context of the entire pseudoknot-containing region. Thus, this work provides a detailed in vitro characterization of the thermodynamic features of the S. cerevisiae TLC1 pseudoknot region for comparison with other telomerase RNA pseudoknots.
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