ArticleOncogene2013
P21-activated kinase 4 (PAK4) is required for metaphase spindle positioning and anchoring.
Article in Oncogene, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 40 citations in OpenAlex.
- PAK4 promotes the cytoskeletal organization and meiotic maturation via phosphorylating DDX17 in oocyte.Cell communication and signaling : CCS · 2025Article
- PAK4-relevant proliferation reduced by cell autophagy via p53/mTOR/p-AKT signaling.Translational cancer research · 2023Article
- Fine-tuning cell organelle dynamics during mitosis by small GTPases.Frontiers of medicine · 2022Review
- The significance of PAK4 in signaling and clinicopathology: A review.Open life sciences · 2022Review
- PAK6 promotes cervical cancer progression through activation of the Wnt/β-catenin signaling pathway.Oncology letters · 2020Article
- Development and Patterning of the Cochlea: From Convergent Extension to Planar Polarity.Cold Spring Harbor perspectives in medicine · 2020Review
- Nonconserved miR-608 suppresses prostate cancer progression through RAC2/PAK4/LIMK1 and BCL2L1/caspase-3 pathways by targeting the 3'-UTRs of RAC2/BCL2L1 and the coding region of PAK4.Cancer medicine · 2019Article
- PAK4 Regulates Actin and Microtubule Dynamics during Meiotic Maturation in Mouse Oocyte.International journal of biological sciences · 2019Article
- PATL2 is a key actor of oocyte maturation whose invalidation causes infertility in women and mice.EMBO molecular medicine · 2018Article
- Identification of the PAK4 interactome reveals PAK4 phosphorylation of N-WASP and promotion of Arp2/3-dependent actin polymerization.Oncotarget · 2017Article
- Palladin is a novel microtubule-associated protein responsible for spindle orientation.Scientific reports · 2017Article
- Review
- Heterogeneity in sarcoma cell lines reveals enhanced motility of tetraploid versus diploid cells.Oncotarget · 2017Article
- Review
- Potential role of p21 Activated Kinase 1 (PAK1) in the invasion and motility of oral cancer cells.BMC cancer · 2016Article
- PAK4 Methylation by SETD6 Promotes the Activation of the Wnt/β-Catenin Pathway.The Journal of biological chemistry · 2016Article
- Group II p21-activated kinases as therapeutic targets in gastrointestinal cancer.World journal of gastroenterology · 2016Review
- ASK1 controls spindle orientation and positioning by phosphorylating EB1 and stabilizing astral microtubules.Cell discovery · 2016Article
- Regulation of NDR1 activity by PLK1 ensures proper spindle orientation in mitosis.Scientific reports · 2015Article
- microRNA-126 suppresses PAK4 expression in ovarian cancer SKOV3 cells.Oncology letters · 2015Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The oncogenic kinase PAK4 was recently found to be involved in the regulation of the G1 phase and the G2/M transition of the cell cycle. We have also identified that PAK4 regulates Ran GTPase activity during mitosis. Here, we show that after entering mitosis, PAK4-depleted cells maintain a prolonged metaphase-like state. In these cells, chromosome congression to the metaphase plate occurs with normal kinetics but is followed by an extended period during which membrane blebbing and spindle rotation are observed. These bipolar PAK4-depleted metaphase-like spindles have a defective astral microtubule (MT) network and are not centered in the cell but are in close contact with the cell cortex. As the metaphase-like state persists, centrosome fragmentation occurs, chromosomes scatter from the metaphase plate and move toward the spindle poles with an active spindle assembly checkpoint, a phenotype that is reminiscent of cohesion fatigue. PAK4 also regulates the acto-myosin cytoskeleton and we report that PAK4 depletion results in the induction of cortical membrane blebbing during prometaphase arrest. However, we show that membrane blebs, which are strongly enriched in phospho-cofilin, are not responsible for the poor anchoring of the spindle. As PAK4 depletion interferes with the localization of components of the dynein/dynactin complexes at the kinetochores and on the astral MTs, we propose that loss of PAK4 could induce a change in the activities of motor proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.