ArticleThe Journal of infectious diseases2012
Targeting pan-resistant bacteria with antibodies to a broadly conserved surface polysaccharide expressed during infection.
Article in The Journal of infectious diseases, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 55 citations in OpenAlex.
- Specific imaging of bacterial infection: a translational approach using positron emission tomography and gallium-68-labeled maltohexaose.Theranostics · 2026Article
- Poly-β-D-(1,6)-N-acetyl-glucosamine (PNAG) glycan vaccines with broad spectrum neutralizing activities.Nature communications · 2025Article
- Bacterial biofilms in the human body: prevalence and impacts on health and disease.Frontiers in cellular and infection microbiology · 2023Review
- The Airway Microbiome and Metabolome in Preterm Infants: Potential Biomarkers of Bronchopulmonary Dysplasia.Frontiers in pediatrics · 2022Article
- Evolution towards Virulence in amBio · 2021Article
- Immunization and Immunotherapy Approaches againstVaccines · 2021Review
- Structural investigation of human S. aureus-targeting antibodies that bind wall teichoic acid.mAbs · 2018Article
- Antibody to Poly-N-acetyl glucosamine provides protection against intracellular pathogens: Mechanism of action and validation in horse foals challenged with Rhodococcus equi.PLoS pathogens · 2018Article
- Phenotypic Variation during Biofilm Formation: Implications for Anti-Biofilm Therapeutic Design.Materials (Basel, Switzerland) · 2018Review
- An Oxygen-Sensing Two-Component System in the Burkholderia cepacia Complex Regulates Biofilm, Intracellular Invasion, and Pathogenicity.PLoS pathogens · 2017Article
- The exceptionally broad-based potential of active and passive vaccination targeting the conserved microbial surface polysaccharide PNAG.Expert review of vaccines · 2016Review
- Extended-spectrum antibodies protective against carbapenemase-producing Enterobacteriaceae.The Journal of antimicrobial chemotherapy · 2016Article
- Identification of Poly-N-acetylglucosamine as a Major Polysaccharide Component of the Bacillus subtilis Biofilm Matrix.The Journal of biological chemistry · 2015Article
- Bacterial Extracellular Polysaccharides in Biofilm Formation and Function.Microbiology spectrum · 2015Review
- Infections Caused by Stenotrophomonas maltophilia in Recipients of Hematopoietic Stem Cell Transplantation.Frontiers in oncology · 2014Review
- Will there ever be a universal Staphylococcus aureus vaccine?Human vaccines & immunotherapeutics · 2013Article
- Antibody to a conserved antigenic target is protective against diverse prokaryotic and eukaryotic pathogens.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- The K1 capsular polysaccharide from Acinetobacter baumannii is a potential therapeutic target via passive immunization.Infection and immunity · 2013Article
- Allosteric activation of exopolysaccharide synthesis through cyclic di-GMP-stimulated protein-protein interaction.The EMBO journal · 2013Article
- A comprehensive analysis of in vitro and in vivo genetic fitness of Pseudomonas aeruginosa using high-throughput sequencing of transposon libraries.PLoS pathogens · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
21 authors at 7 institutions in 1 country.
Funding
Abstract
backgroundNew therapeutic targets for antibiotic-resistant bacterial pathogens are desperately needed. The bacterial surface polysaccharide poly-β-(1-6)-N-acetyl-glucosamine (PNAG) mediates biofilm formation by some bacterial species, and antibodies to PNAG can confer protective immunity. By analyzing sequenced genomes, we found that potentially multidrug-resistant bacterial species such as Klebsiella pneumoniae, Enterobacter cloacae, Stenotrophomonas maltophilia, and the Burkholderia cepacia complex (BCC) may be able to produce PNAG. Among patients with cystic fibrosis patients, highly antibiotic-resistant bacteria in the BCC have emerged as problematic pathogens, providing an impetus to study the potential of PNAG to be targeted for immunotherapy against pan-resistant bacterial pathogens.
methodsThe presence of PNAG on BCC was assessed using a combination of bacterial genetics, microscopy, and immunochemical approaches. Antibodies to PNAG were tested using opsonophagocytic assays and for protective efficacy against lethal peritonitis in mice.
resultsPNAG is expressed in vitro and in vivo by the BCC, and cystic fibrosis patients infected by the BCC species B. dolosa mounted a PNAG-specific opsonophagocytic antibody response. Antisera to PNAG mediated opsonophagocytic killing of BCC and were protective against lethal BCC peritonitis even during coinfection with methicillin-resistant Staphylococcus aureus.
conclusionsOur findings raise potential new therapeutic options against PNAG-producing bacteria, including even pan-resistant pathogens.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.