ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2012
Molecular mechanisms in the dramatic enhancement of HIV-1 Tat transduction by cationic liposomes.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- Exploring CPP Enhanced Hypoxia Targeting: An Approach Using Radiolabeled 2-nitroimidazole TAT Conjugate.Drug development research · 2026Article
- HIV-1C and HIV-1B Tat protein polymorphism in Southern Brazil.Journal of neurovirology · 2021Observational
- Productive HIV infection in astrocytes can be established via a nonclassical mechanism.AIDS (London, England) · 2020Article
- Two-pore channels regulate Tat endolysosome escape and Tat-mediated HIV-1 LTR transactivation.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- BK channels regulate extracellular Tat-mediated HIV-1 LTR transactivation.Scientific reports · 2019Article
- Apolipoprotein E isoform dependently affects Tat-mediated HIV-1 LTR transactivation.Journal of neuroinflammation · 2018Article
- HIV Tat protein and amyloid-β peptide form multifibrillar structures that cause neurotoxicity.Nature structural & molecular biology · 2017Article
- Exosome-associated release, uptake, and neurotoxicity of HIV-1 Tat protein.Journal of neurovirology · 2016Article
- Rationale and Methodology of Reprogramming for Generation of Induced Pluripotent Stem Cells and Induced Neural Progenitor Cells.International journal of molecular sciences · 2016Review
- Intracellular Delivery of Molecular Cargo Using Cell-Penetrating Peptides and the Combination Strategies.International journal of molecular sciences · 2015Review
- Article
- Enhanced cellular entry and efficacy of tat conjugates by rational design of the auxiliary segment.Molecular pharmaceutics · 2014Article
- Proteins reprogramming: present and future.TheScientificWorldJournal · 2012Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Human immunodeficiency virus type 1 (HIV-1) transactivator of transcription (Tat) protein possesses a unique membrane-transduction property. Interestingly, Tat transduction could be dramatically increased 1000-fold based on LTR-transactivation assay when complexed with cationic liposomes (lipo-Tat), compared with Tat alone. Therefore, underlining mechanisms were explored further. Microscopy and flow cytometry showed that this effect was associated with enhanced membrane binding, large particle formation (1-2 μm) and increased intracellular uptake of Tat fluorescent proteins. Using pharmacological assays and immune colocalizations, it was found that lipid raft-dependent endocytosis and macropinocytosis were major pathways involved in lipo-Tat uptake, and actin-filaments played a major role in intracellular trafficking of lipo-Tat to the nucleus. Furthermore, we found that the Tat hydrophobic domain (aa 36-47) mediated formation of two positively charged molecules into lipo-Tat complexes via hydrophobic bonds, based on LTR-transactivation inhibition assay. Thus, the hydrophobic domain may play an important role in Tat protein uptake and be useful for intracellular delivery of biomacromolecules if coupled together with Tat basic peptide, a cell-penetrating peptide.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.