ArticleAmerican journal of physiology. Heart and circulatory physiology2012
Flow mechanotransduction regulates traction forces, intercellular forces, and adherens junctions.
Article in American journal of physiology. Heart and circulatory physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 85 citations in OpenAlex.
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- Senescent endothelial cells: a potential target for diabetic retinopathy.Angiogenesis · 2024Review
- A homeostatic role of nucleus-actin filament coupling in the regulation of cellular traction forces in fibroblasts.Biomechanics and modeling in mechanobiology · 2024Article
- Transient low shear-stress preconditioning influences long-term endothelial traction and alignment under high shear flow.American journal of physiology. Heart and circulatory physiology · 2024Article
- Low or oscillatory shear stress and endothelial permeability in atherosclerosis.Frontiers in physiology · 2024Review
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- Human endothelial cells display a rapid tensional stress increase in response to tumor necrosis factor-α.PloS one · 2022Article
- Potential Therapeutic Applications of N-Cadherin Antagonists and Agonists.Frontiers in cell and developmental biology · 2022Review
- Revisiting tissue tensegrity: Biomaterial-based approaches to measure forces across length scales.APL bioengineering · 2021Review
- Never Change a Flowing System? The Effects of Retrograde Flow on Isolated Perfused Lungs and Vessels.Cells · 2021Article
- (De)form and Function: Measuring Cellular Forces with Deformable Materials and Deformable Structures.Advanced healthcare materials · 2020Review
- The effect of shear stress reduction on endothelial cells: A microfluidic study of the actin cytoskeleton.Biomicrofluidics · 2020Article
- The Importance of Mechanical Forces forFrontiers in physiology · 2020Review
- The Interplay Between Cell-Cell and Cell-Matrix Forces Regulates Cell Migration Dynamics.Biophysical journal · 2019Article
- Article
- Biophysics of Cell-Substrate Interactions Under Shear.Frontiers in cell and developmental biology · 2019Review
- Blood Brothers: Hemodynamics and Cell-Matrix Interactions in Endothelial Function.Antioxidants & redox signaling · 2016Review
- Multiplexed Fluid Flow Device to Study Cellular Response to Tunable Shear Stress Gradients.Annals of biomedical engineering · 2016Article
- Review of cellular mechanotransduction on micropost substrates.Medical & biological engineering & computing · 2016Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Endothelial cells respond to fluid shear stress through mechanotransduction responses that affect their cytoskeleton and cell-cell contacts. Here, endothelial cells were grown as monolayers on arrays of microposts and exposed to laminar or disturbed flow to examine the relationship among traction forces, intercellular forces, and cell-cell junctions. Cells under laminar flow had traction forces that were higher than those under static conditions, whereas cells under disturbed flow had lower traction forces. The response in adhesion junction assembly matched closely with changes in traction forces since adherens junctions were larger in size for laminar flow and smaller for disturbed flow. Treating the cells with calyculin-A to increase myosin phosphorylation and traction forces caused an increase in adherens junction size, whereas Y-27362 cause a decrease in their size. Since tugging forces across cell-cell junctions can promote junctional assembly, we developed a novel approach to measure intercellular forces and found that these forces were higher for laminar flow than for static or disturbed flow. The size of adherens junctions and tight junctions matched closely with intercellular forces for these flow conditions. These results indicate that laminar flow can increase cytoskeletal tension while disturbed flow decreases cytoskeletal tension. Consequently, we found that changes in cytoskeletal tension in response to shear flow conditions can affect intercellular tension, which in turn regulates the assembly of cell-cell junctions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.