ArticleJournal of clinical pathology2012
Utility of multispectral imaging in automated quantitative scoring of immunohistochemistry.
Article in Journal of clinical pathology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
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- A neutrophil extracellular traps-related classification predicts prognosis and response to immunotherapy in colon cancer.Scientific reports · 2023Article
- Leveraging mid-infrared spectroscopic imaging and deep learning for tissue subtype classification in ovarian cancer.The Analyst · 2023Article
- Conditional Knockout of Hypoxia-Inducible Factor 1-Alpha in Tumor-Infiltrating Neutrophils Protects against Pancreatic Ductal Adenocarcinoma.International journal of molecular sciences · 2023Article
- Transducin β-like protein 1 controls multiple oncogenic networks in diffuse large B-cell lymphoma.Haematologica · 2021Article
- CD30Cancer immunology, immunotherapy : CII · 2021Article
- Comparative Analysis of Immunohistochemical Staining Intensity Determined by Light Microscopy, ImageJ and QuPath in Placental Hofbauer Cells.Acta histochemica et cytochemica · 2021Article
- Multiomic analysis and immunoprofiling reveal distinct subtypes of human angiosarcoma.The Journal of clinical investigation · 2020Article
- Immunohistochemical scoring of CD38 in the tumor microenvironment predicts responsiveness to anti-PD-1/PD-L1 immunotherapy in hepatocellular carcinoma.Journal for immunotherapy of cancer · 2020Article
- Hyperspectral and multispectral imaging in digital and computational pathology: a systematic review [Invited].Biomedical optics express · 2020Review
- Overview of multiplex immunohistochemistry/immunofluorescence techniques in the era of cancer immunotherapy.Cancer communications (London, England) · 2020Review
- Digital image analysis of multiplex fluorescence IHC in colorectal cancer recognizes the prognostic value of CDX2 and its negative correlation with SOX2.Laboratory investigation; a journal of technical methods and pathology · 2020Article
- The role of Ki-67 in Asian triple negative breast cancers: a novel combinatory panel approach.Virchows Archiv : an international journal of pathology · 2019Article
- Prognostic value of CD8 + PD-1+ immune infiltrates and PDCD1 gene expression in triple negative breast cancer.Journal for immunotherapy of cancer · 2019Article
- Expression of CD38 on Macrophages Predicts Improved Prognosis in Hepatocellular Carcinoma.Frontiers in immunology · 2019Article
- Principles and approaches for reproducible scoring of tissue stains in research.Laboratory investigation; a journal of technical methods and pathology · 2018Review
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Authors and funding
12 authors.
Funding
Abstract
backgroundAutomated scanning devices and image analysis software provide a means to overcome the limitations of manual semiquantitative scoring of immunohistochemistry. Common drawbacks to automated imaging systems include an inability to classify tissue type and an inability to segregate cytoplasmic and nuclear staining.
methodsImmunohistochemistry for the membranous marker α-catenin, the cytoplasmic marker stathmin and the nuclear marker Ki-67 was performed on tissue microarrays (TMA) of archival formalin-fixed paraffin-embedded tissue comprising 471 (α-catenin and stathmin) and 511 (Ki-67) cases of prostate adenocarcinoma. These TMA were quantitatively analysed using two commercially available automated image analysers, the Ariol SL-50 system and the Nuance system from CRi. Both systems use brightfield microscopy for automated, unbiased and standardised quantification of immunohistochemistry, while the Nuance system has spectral deconvolution capabilities.
resultsOverall concordance between scores from both systems was excellent (r=0.90; 0.83-0.95). The software associated with the multispectral imager allowed accurate automated classification of tissue type into epithelial glandular structures and stroma, and a single-step segmentation of staining into cytoplasmic or nuclear compartments allowing independent evaluation of these areas. The Nuance system, however, was not able to distinguish reliably between tumour and non-tumour tissue. In addition, variance in the labour and time required for analysis between the two systems was also noted.
conclusionDespite limitations, this study suggests some beneficial role for the use of a multispectral imaging system in automated analysis of immunohistochemistry.
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